High density of REC8 constrains sister chromatid axes and prevents illegitimate synaptonemal complex formation
High density of REC8 constrains sister chromatid axes and prevents illegitimate synaptonemal complex formation
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DOI：
10.15252/embr.201642030
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发表时间：
2016-06-01
期刊：
影响因子：
7.7
通讯作者：
Hoog, Christer
中科院分区：
文献类型：
--
作者：
Agostinho, Ana;Manneberg, Otto;Hoog, Christer
在减数分裂过程中，凝聚素复合体介导姐妹染色单体凝聚（SCC）、联会复合体（SC）组装和联会。在这里，使用超分辨率显微镜，我们成像的姐妹染色单体轴在小鼠的性母细胞，具有正常或降低水平的粘附复合物，评估定位的粘附复合物，SCC和SC形成之间的关系。我们发现，REC 8焦点彼此分开的距离小于15%的总染色体轴长度在野生型性母细胞。降低水平的凝聚素复合物导致姐妹染色单体轴向元件（LSAE）的局部分离，以及在这些网站的非法SC的形成。REC 8而不是RAD 21或RAD 21 L粘附素复合物位于LSAE的侧翼位点，而RAD 21和RAD 21 L主要沿着分离的姐妹染色单体轴出现。基于这些观察和REC 8沿着姐妹染色单体轴的定量分布分析，我们提出随机分布的REC 8粘附素复合物的高密度促进SCC和防止非法SC形成。
During meiosis, cohesin complexes mediate sister chromatid cohesion (SCC), synaptonemal complex (SC) assembly and synapsis. Here, using super-resolution microscopy, we imaged sister chromatid axes in mouse meiocytes that have normal or reduced levels of cohesin complexes, assessing the relationship between localization of cohesin complexes, SCC and SC formation. We show that REC8 foci are separated from each other by a distance smaller than 15% of the total chromosome axis length in wild-type meiocytes. Reduced levels of cohesin complexes result in a local separation of sister chromatid axial elements (LSAEs), as well as illegitimate SC formation at these sites. REC8 but not RAD21 or RAD21L cohesin complexes flank sites of LSAEs, whereas RAD21 and RAD21L appear predominantly along the separated sister-chromatid axes. Based on these observations and a quantitative distribution analysis of REC8 along sister chromatid axes, we propose that the high density of randomly distributed REC8 cohesin complexes promotes SCC and prevents illegitimate SC formation.