Nicotinamide Adenine Dinucleotide Phosphate/Neuronal Nitric Oxide Synthase-Positive Neurons in the Trigeminal Subnucleus Caudalis Involved in Tooth Pulp Nociception
Nicotinamide Adenine Dinucleotide Phosphate/Neuronal Nitric Oxide Synthase-Positive Neurons in the Trigeminal Subnucleus Caudalis Involved in Tooth Pulp Nociception
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DOI：
10.1002/jnr.22676
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发表时间：
2011-09-01
影响因子：
4.2
通讯作者：
Rios, Hugo
中科院分区：
文献类型：
--
作者：
Canzobre, Mariela C.;Rios, Hugo
面部结构的感觉信息，包括牙髓、牙周组织和牙龈，在三叉神经复合体中传递。牙髓炎症是临床常见的问题，这种外周损伤可引起三叉神经伤害性神经元的神经可塑性改变。有相当多的证据表明，三叉神经尾侧亚核（Vc）是三叉神经伤害性信息的主要中继以及疼痛刺激的调制。支配牙髓的谷氨酸能初级传入投射到Vc的最浅层。N-甲基-D-天冬氨酸受体刺激导致一氧化氮合酶（NOS）的激活，其合成自由基一氧化氮（NO）。该酶主要表达于Vc的第II层中间神经元，第I层中的少量细胞以及深层投射神经元中。在本研究中，我们分析了神经元型一氧化氮合酶（nNOS）的时间变化后，在Vc局部回路单侧中间磨牙牙髓损伤。我们的研究结果表明，周围牙髓损伤导致nNOS酶的相对量和活性的神经可塑性变化。此外，一段时间后，氮能系统转移到初始值，独立于牙髓组织中的炎症的持续性。(C)2011 Wiley-Liss，Inc.
Sensory information on facial structures, including teeth pulp, periodontium, and gingiva, is relayed in the trigeminal complex. Tooth pulp inflammation constitutes a common clinical problem, and this peripheral injury can induce neuroplastic changes in trigeminal nociceptive neurons. There is considerable evidence that the trigeminal subnucleus caudalis (Vc) is the principal relay for trigeminal nociceptive information as well as modulation of the painful stimuli. Glutamatergic primary afferents innervating the tooth pulp project to the most superficial laminae of the Vc. N-metthyl-D-aspartate receptor stimulation leads to the activation of the enzyme nitric oxide synthase (NOS), which synthesizes the free radical nitric oxide (NO). This enzyme is expressed mainly in lamina II interneurons, and in a small number of cells in lamina I as well as in deep laminae projection neurons of Vc. In the present study, we analyzed the temporal changes in neuronal NOS (nNOS) in Vc local circuitries after unilateral intermediate molar pulp injury. Our results demonstrate that a peripheral dental pulp injury leads to neuroplastic changes in the relative amount and activity of nNOS enzyme. Moreover, after a period of time, the nitrergic system shifts to the initial values, independently of the persistence of inflammation in the pulp tissues. (C) 2011 Wiley-Liss, Inc.