MS-based conformation analysis of recombinant proteins in design, optimization and development of biopharmaceuticals
MS-based conformation analysis of recombinant proteins in design, optimization and development of biopharmaceuticals
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DOI：
10.1016/j.ymeth.2018.04.011
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发表时间：
2018-07-15
期刊：
影响因子：
4.8
通讯作者：
Zhang, Zhongqi
中科院分区：
文献类型：
--
作者：
Goswami, Devrishi;Zhang, Jun;Zhang, Zhongqi
用于分析蛋白质高级结构的基于质谱（MS）的方法在生物制药开发领域中获得了越来越多的应用。该领域使用的主要方法包括天然MS、氢氘交换-MS、共价标记、交联和有限蛋白水解。本文将简要介绍这些基于MS的方法，然后讨论这些方法如何在蛋白质疗法的发现和开发的不同阶段做出贡献。
Mass spectrometry (MS)-based methods for analyzing protein higher order structures have gained increasing application in the field of biopharmaceutical development. The predominant methods used in this area include native MS, hydrogen deuterium exchange-MS, covalent labeling, cross-linking and limited proteolysis. These MS based methods will be briefly described in this article, followed by a discussion on how these methods contribute at different stages of discovery and development of protein therapeutics.