Gene induction and categorical reprogramming during in vitro human endometrial fibroblast decidualization
Gene induction and categorical reprogramming during in vitro human endometrial fibroblast decidualization
复制标题
DOI：
10.1152/physiolgenomics.00061.2001
复制
发表时间：
2001-12-01
影响因子：
4.6
通讯作者：
Aronow, BJ
中科院分区：
文献类型：
--
作者：
Brar, AK;Handwerger, S;Aronow, BJ
人类蜕膜成纤维细胞在一个被称为蜕膜化的过程中，经历了获得内分泌、代谢和结构细胞功能的分化承诺。脱个体化对胚胎着床和胎盘功能至关重要。我们通过微阵列分析表征了蜕膜成纤维细胞分化过程中的基因表达模式动力学。在所分析的6918个基因中，有121个基因被双相诱导，110个基因被下调，50个基因表现出双相行为。动态调控基因可分为9个基于K均值算法的动力学模式组，并按生物学分类分为5类：细胞和组织功能、细胞和组织结构、基因表达调控、表达序列标签（EST）和“功能未知”。特定功能群和基因家族内的基因重编程是一个突出的特征，包括同时诱导和下调一组具有相关功能的基因。我们之前在胎儿滋养细胞分化过程中观察到概念上类似的过程，其中相同的现象适用于不同的基因。在两种模式调控的569个动态调控基因中，只有81个是共同的。这些结果表明，在重点功能类别内基因表达的重编程代表了细胞分化的一个基本方面。
Human decidual fibroblasts undergo a differentiative commitment to the acquisition of endocrine, metabolic, and structural cell functions in a process known as decidualization. Decidualization is critical for embryo implantation and placental function. We characterized gene expression pattern kinetics during decidual fibroblast differentiation by microarray analysis. Of 6,918 genes analyzed, 121 genes were induced by more than twofold, 110 were downregulated, and 50 showed biphasic behavior. Dynamically regulated genes were could be fit into nine K- means algorithm- based kinetic pattern groups, and by biologic classification, into five categories: cell and tissue function, cell and tissue structure, regulation of gene expression, expressed sequence tag (EST), and "function unknown." Reprogramming of genes within specific functional groups and gene families was a prominent feature that consisted of simultaneous induction and downregulation of a set of genes with related function. We previously observed a conceptually similar process during fetal trophoblast differentiation, in which the same phenomena applied to different genes. Of the 569 dynamically regulated genes regulated by either model, only 81 of these were in common. These results suggest that reprogramming of gene expression within focused functional categories represents a fundamental aspect of cellular differentiation.