Cytosolic free calcium in chick heart cells. Its role in cell injury.
Cytosolic free calcium in chick heart cells. Its role in cell injury.
复制标题
鸡心脏细胞中的胞质游离钙。
DOI：
10.1016/s0022-2828(85)80005-5
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发表时间：
1985
影响因子：
5
通讯作者：
Lieberman,M
中科院分区：
文献类型：
--
作者：
Murphy,E;Jacob,R;Lieberman,M
使用两种测量新鲜解聚的胚胎鸡心脏细胞 Cafin 的方法，研究了胞质游离 Ca2+ (Caf) 在细胞损伤中的作用。使用偶氮胂 III 的零点法基于确定当质膜通透性增加时不发生净 Ca2+ 移动时的细胞外 Ca2+ 浓度。采用该技术，在基础状态下零点Cafaveraging 0.23±0.07 μm(n=6)。使用细胞内荧光染料quin2测量Cafa值，得到0.05±0.01μm（n=5）。通过胞质酶 LDH 的释放来测量，各种药物的 Cafby 升高与细胞损伤的增加有关。然而，在所有实验条件下，Cafis 和 LDH 释放之间的关系并不是直接的，这表明 Cafis 的水平并不是细胞损伤的唯一决定因素。
The role of cytosolic free Ca2+(Caf) in cell injury was investigated using two methods for measuring Cafin freshly disaggregated embryonic chick heart cells. The null-point method, using arsenazo III, is based on determining the extracellular Ca2+concentration at which no net Ca2+movement occurs when plasma membrane permeability is increased. With this technique, the null point Cafaveraged 0.23±0.07 μm(n=6) in the basal state. Using quin2, an intracellular fluorescent dye, to measure Cafa value of 0.05±0.01 μm(n=5) was obtained. Elevation of Cafby various agents was associated with an increase in cell injury as measured by the release of the cytosolic enzyme, LDH. However, the relationship between Cafand LDH release was not a direct one under all experimental conditions, indicating that the level of Cafis not the sole determinant of cell injury.