HUMAN EMBRYONAL CARCINOMA-CELLS IN CULTURE DO NOT SYNTHESIZE FIBRONECTIN UNTIL THEY DIFFERENTIATE
HUMAN EMBRYONAL CARCINOMA-CELLS IN CULTURE DO NOT SYNTHESIZE FIBRONECTIN UNTIL THEY DIFFERENTIATE
复制标题
DOI：
10.1002/ijc.2910300506
复制
发表时间：
1982-01-01
影响因子：
6.4
通讯作者：
ANDREWS, PW
中科院分区：
文献类型：
--
作者：
ANDREWS, PW
研究了 2102Ep（一种源自睾丸生殖细胞肿瘤的人类细胞系）克隆的纤连蛋白表达。在高密度培养物中，这些细胞保留胚胎癌表型并且不表达细胞表面抗原SSEA-I。它们不合成纤连蛋白。然而，当在低密度下生长时，许多细胞出现分化，如其形态和 SSEA-I 表达的变化所表明的。此类培养物合成纤连蛋白。此外，当通过荧光激活细胞分选将低密度培养物分为SSEA-I阳性和SSEA-I阴性亚群时，纤连蛋白合成仅限于SSEA-I阳性细胞。这些细胞产生的纤连蛋白的表观分子量 (261,000) 略大于二倍体成纤维细胞产生的分子量 (250,000)，并且可能代表胚胎形式。它不是作为细胞外基质形成的，并且不能通过细胞单层的免疫荧光分析来检测。
The expression of fibronectin by clones of 2102Ep, a human cell line derived from a testicular germ-cell tumor, was studied. In high-density cultures these cells retain an embryonal carcinoma phenotype and do not express the cell surface antigen SSEA-I. They do not synthesize fibronectin. However, when grown at low densities, many of the cells appear to differentiate, as indicated by a change in their morphology and their expression of SSEA-I. Such cultures synthesize fibronectin. Further, when low-density cultures were fractionated into SSEA-I-positive and SSEA-I-negative subpopulations by fluorescence-activated cell sorting, fibronectin synthesis was confined to the SSEA-I-positive cells. The fibronectin produced by these cells exhibits an apparent MW (261,000) slightly greater than that produced by diploid fibroblasts (250,000) and may represent an embryonic form. It is not laid down as an extracellular matrix and cannot be detected by immunofluorescence analysis of cell monolayers.