Appearance and concentrations of catecholamines and their biosynthesis in the embryonic and developing chick.
Appearance and concentrations of catecholamines and their biosynthesis in the embryonic and developing chick.
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发表时间：
1968
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通讯作者：
L. Ignarro;F. E. Shideman
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作者：
L. Ignarro;F. E. Shideman
本文测定了多巴（DA）、多巴胺、去甲肾上腺素和肾上腺素在鸡胚和鸡胚心脏中的出现时间和浓度。DA和多巴胺首先在心脏中检测到的第4和第6天的孵化，分别。在孵育第3天首次检测到心脏去甲肾上腺素和肾上腺素。心脏中这些儿茶酚胺和DA的内源性水平在整个胚胎发育过程中和孵化后的短时间内波动。在开发的剩余时间内，其浓度没有进一步的显著变化。在整个胚胎中，DA和多巴胺分别在第1天和第2天首次被检测到。第3天首次检测到去甲肾上腺素和肾上腺素。由于这些儿茶酚在整个胚胎中似乎有某种有序的顺序出现，根据其生物合成的方案，确定了负责其合成的每种酶的出现顺序。酪氨酸羟化酶、多巴脱羧酶、多巴胺β-氧化酶和苯乙醇胺-N-甲基转移酶分别在培养的第1、2、4和6天首次检测到活性。
The times of appearance and concentrations of dopa (DA), dopamine, norepinephrine and epinephrine were determined in the embryo and in the heart of the embryonic and developing chick. DA and dopamine were first detected in the heart on the 4th and 6th days of incubation, respectively. Cardiac norepinephrine and epinephrine were first detected on the 3rd day of incubation. The endogenous levels of these catecholamines and DA in the heart fluctuated throughout embryonic development and for a short time after hatching. During the remainder of development there were no further marked changes in their concentrations. In the whole embryo, DA and dopamine were first detected on the 1st and 2nd days, respectively. Norepinephrine and epinephrine were first detected on the 3rd day. As there appeared to be a somewhat orderly sequential appearance of these catechols, in the whole embryo, in accordance with the scheme for their biosynthesis, the order of appearance of each of the enzymes responsible for their synthesis was determined. Tyrosine hydroxylase, dopa decarboxylase, dopamine β-oxidase and phenylethanolamine-N-methyl transferase activities were first detected on the 1st, 2nd, 4th and 6th days of incubation, respectively.