Effect of dietary supplementation of prebiotics and probiotics on intestinal microarchitecture in broilers reared under cyclic heat stress
Effect of dietary supplementation of prebiotics and probiotics on intestinal microarchitecture in broilers reared under cyclic heat stress
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DOI：
10.1111/jpn.12041
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发表时间：
2013-05-01
影响因子：
2.7
通讯作者：
Rehman, H.
中科院分区：
文献类型：
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作者：
Ashraf, S.;Zaneb, H.;Rehman, H.
本研究旨在评估膳食补充益生元、甘露寡糖 (MOS) 和/或益生菌 (LBP) 对周期性热应激饲养的肉鸡上皮内淋巴细胞 (IEL) 计数、杯状细胞 (GC) 计数和分化以及肠道微结构的影响。日龄肉鸡（n=250）随机分为五组。五十只鸡在热中性区（TNZ）内饲养。其余的鸡从第 21 天到第 42 天遭受循环热应激（35 摄氏度，75% 相对湿度，8 小时/天）。这些鸡饲喂以玉米-大豆为基础的基础日粮，或在同一日粮中添加 0.5% MOS (HS-MOS) 或 0.1% LBP (HS-LBP) 或其组合 (HS-SYN)。在第42天宰杀家禽。从十二指肠中部、空肠和回肠收集组织样本，并用苏木精和伊红或联合阿尔新蓝和PAS技术染色。所有差异在 p 时均被认为是显着的
This study was designed to evaluate the effect of dietary supplementation of prebiotics, mannanoligosaccharides (MOS) and/or probiotics (LBP) on intraepithelial lymphocytes (IEL) count, goblet cells (GC) count and differentiation and intestinal micro-architecture in broilers reared under cyclic heat stress. Day-old broilers (n=250) were randomly divided into five groups. Fifty birds were reared within the thermoneutral zone (TNZ). Remaining birds were subjected to cyclic heat stress from day 21 to 42 (35 degrees C, 75% RH, 8h/d). The birds were fed corn-soy-based basal diet or the same diet supplemented with 0.5% MOS (HS-MOS), or 0.1% LBP (HS-LBP), or their combination (HS-SYN). The birds were slaughtered on day 42. Tissue samples were collected from mid-duodenum, jejunum and ileum, and stained with haematoxylin and eosin or combined Alcian blue and PAS technique. All differences were considered significant at p