Regulation of dopamine uptake by basic fibroblast growth factor and epidermal growth factor in cultured rat astrocytes
Regulation of dopamine uptake by basic fibroblast growth factor and epidermal growth factor in cultured rat astrocytes
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DOI：
10.1016/s0168-0102(99)00053-x
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发表时间：
1999-09-01
影响因子：
2.9
通讯作者：
Matsumiya, T
中科院分区：
文献类型：
--
作者：
Inazu, M;Takeda, H;Matsumiya, T
观察培养的大鼠星形胶质细胞对多巴胺(DA)摄取的特点以及碱性成纤维细胞生长因子(BFGF)和表皮生长因子(EGF)等神经营养因子对DA摄取的调节作用。在单胺氧化酶(MAO)和儿茶酚-O-甲基转移酶(COMT)抑制剂存在下，星形胶质细胞通过对温度降低敏感的Na+依赖和非Na+依赖机制摄取DA。Na+依赖组分和Na+非依赖组分随[H-3]DA浓度的增加呈线性增加(1-1000mU/M)，未表现出饱和状态。Na+-K+-ATPase抑制剂哇巴因显著抑制Na+依赖的DA摄取。在碱性成纤维细胞生长因子处理的星形胶质细胞中，[H-3]DA摄取呈时间依赖性增加，直到48h，在Na+存在和不存在的情况下，72h后[H-3]DA摄取均下降。在EGF处理的星形胶质细胞中，无论有无Na+，[H-3]DA摄取均呈时间依赖性增加，直至72 h。放线菌素D、放线菌素D、放线菌素A、放线菌素D和灯盏花素A可显著抑制EGF或bFGF对DA摄取的促进作用。这些结果提示，培养的大鼠星形胶质细胞存在Na+依赖和非Na+依赖的DA摄取，EGF或bFGF可能刺激神经元外DA转运体的表达和转位。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
We examined the characteristics of dopamine (DA) uptake and its regulation by neurotrophic factors such as basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in cultured rat astrocytes. In the presence of inhibitors of monoamine oxidase (MAO) and catechol-O-methyl-transferase (COMT), astrocytes took up DA by Na+-dependent and Na+-independent mechanisms that were sensitive to a reduction in temperature. The Na+-dependent and Na+-independent components increased linearly with increasing [H-3]DA concentration (1-1000 mu M), and showed no saturation. Na+-dependent DA uptake was significantly inhibited by ouabain, a Na+-K+ ATPase inhibitor. In bFGF-treated astrocytes, [H-3]DA uptake increased in a time-dependent manner until 48 h, and declined after 72 h in both the presence and absence of Na+. In EGF-treated astrocytes, [H-3]DA uptake increased in a time-dependent manner until 72 h in both the presence and absence of Na+. This enhancement of DA uptake induced by EGF or bFGF was significantly inhibited when the cells were cultured with actinomycin D, cycloheximide, or brefeldin A. Actinomycin D and brefeldin A also significantly inhibited the basal uptake of [H-3]DA into astrocytes. These results suggest the existence of Na+-dependent and Na+-independent DA uptake in cultured rat astrocytes, and that EGF or bFGF might stimulate the expression and translocation of the extraneuronal DA transporter. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.