SUSCEPTIBILITY TO ALLOIMMUNIZATION TO PLATELET HPA-1A ANTIGEN INVOLVES TAP1 POLYMORPHISM
SUSCEPTIBILITY TO ALLOIMMUNIZATION TO PLATELET HPA-1A ANTIGEN INVOLVES TAP1 POLYMORPHISM
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DOI：
10.1016/0198-8859(94)90007-8
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发表时间：
1994-10-01
期刊：
影响因子：
2.7
通讯作者：
MULLER, JY
中科院分区：
文献类型：
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作者：
BRAUD, V;CHEVRIER, D;MULLER, JY
血小板减少新生儿的母亲对血小板HPA-1a抗原的同种异体免疫与人类白细胞抗原II类结构(DR3和DR13)密切相关，特别是与人类白细胞抗原-DR52a(98%的病例报告)有关。由于最近在MHC II类区域定位了新的基因，为了更有效地表征参与这种同种免疫的MHC基因，我们采用ARMS-PCR技术对TAP1和TAP2基因的多态性进行了分型。我们的研究结果表明，TAP1*0102等位基因与NAIT的关联仅在人类白细胞抗原-DR13-DR52a免疫妇女(50%)与人类白细胞抗原-DR13-DR52a对照人群(20%)(p<；0.05)中显著相关，而在人类白细胞抗原-DR3-DR52a对照妇女人群中无显著关联。TAP1*0102和DRB1*13等位基因之间不存在连锁不平衡(Delta=-0.0063)，可以解释这一结果。TAP1*0102等位基因在HLADR13-DR52a免疫妇女中的频率较高，提示HPA-1a抗原提呈和识别可能受到非经典的HLAII类基因多态性的影响，或者其他连锁但未知的基因可能会影响HPA-1a的抗原提呈和识别。
The alloimmunization against platelet HPA-la antigen in mothers of thrombocytopenic neonates is strongly associated with HLA class II structures (DR3 and DR13) and especially with HLA-DR52a antigen (98% of the cases reported here). Because new genes have recently been mapped within the MHC class II region, we typed TAP1 and TAP2 gene polymorphisms by ARMS-PCR in order to characterize more effectively MHC genes involved in this alloimmunization. Our re suits showed that TAP1*0102 allele was significantly associated with NAIT only in the population of HLA-DR13-DR52a-immunized women (50%) versus HLA-DR13-DR52a controls (20%) (p < 0.05), and not in HLA-DRS-DRS 2a-immunized women versus HLA-DR3-DR52a controls. There is no linkage disequilibrium between TAP1*0102 and DRB1*13 alleles (Delta = -0.0063) that could account for this result. The higher frequency of TAP1*0102 allele among HLA-DR13-DR52a-immunized women suggests that HPA-1a antigen presentation and recognition may be influenced by nonclassic HLA class II gene polymorphisms, or that other linked but yet unknown genes could interfere.