Attachment of cultured fibroblasts and ultrastructural analysis of simulated cervical resorptions treated with high-power lasers and MTA
Attachment of cultured fibroblasts and ultrastructural analysis of simulated cervical resorptions treated with high-power lasers and MTA
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DOI：
10.1016/j.tripleo.2009.09.021
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发表时间：
2010-02-01
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影响因子：
--
通讯作者：
Lage-Marques, Jose Luiz
中科院分区：
文献类型：
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作者：
Raldi, Denise Pontes;Mello, Isabel;Lage-Marques, Jose Luiz
目标。本研究评估了高功率激光照射和使用三氧化二矿骨料（MTA）后成纤维细胞附着和模拟颈椎根吸收的形态学变化。研究设计。在40颗单根牙的冠状三分之一处做标准化的空腔，根据治疗方法分为4组：(1)未处理对照，(2)MTA, (3) Er:YAG激光（42 mJ, 10 Hz, 10 s）照射，(4)大功率二极管激光（1 W, 10 s）照射。制备切片进行扫描电镜分析，每组6个样品镀人牙龈成纤维细胞，4个样品进行超微结构分析。细胞粘附程度由大到小依次为G3 > G4 > G1 > G2，各组间差异有统计学意义（P < 0.05）。经Er: YAG辐照后，牙本质表面粗糙，无涂抹层，牙本质小管开放；二极管激光照射组牙本质表面光滑，涂抹层多，牙本质小管闭合；对照组牙本质小管封闭，涂片层数较多。Er: YAG和二极管激光照射引起模拟吸收的牙本质表面的形态学变化，有利于细胞的粘附。MTA表现出较低的生物相容性，但能使细胞粘附。（口腔外科口腔医学口腔病理口腔放射学杂志2010；109:e154-e161）
Objective. The present study evaluated the fibroblast attachment and the morphologic changes of simulated cervical root resorptions after irradiation with high-power lasers and the use of mineral trioxide aggregate (MTA).Study design. Standardized cavities were done on the coronal third of 40 single-rooted teeth and assigned into 4 groups according to the treatment applied: ( 1) untreated control, ( 2) use of MTA, ( 3) irradiation with Er:YAG laser (42 mJ, 10 Hz, 10 s), and ( 4) irradiation with high-power diode laser ( 1 W, 10 s). Fragments were prepared for SEM analysis: 6 samples of each group were plated with human gingival fibroblasts and 4 samples were used for ultrastructural analysis.Results. The cells' attachment was in decreasing order: G3 > G4 > G1 > G2, with statistical differences among all groups ( P < .05). The samples irradiated with Er: YAG exhibited roughness on the dentinal surface, no smear layer, and open dentinal tubules; those irradiated with diode laser exhibited a smooth surface, more smear layer, and closed dentinal tubules; and the control group showed high amount of smear layer and closed dentin tubules.Conclusions. Irradiation with Er: YAG and diode lasers caused morphologic changes on the dentinal surfaces of simulated resorptions that favored cells' adhesion. MTA showed lower biocompatibility than irradiated groups but allowed cells' adhesion. ( Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e154-e161)