Cutting edge: CTLs rapidly capture membrane fragments from target cells in a TCR signaling-dependent manner
Cutting edge: CTLs rapidly capture membrane fragments from target cells in a TCR signaling-dependent manner
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DOI：
10.4049/jimmunol.166.6.3645
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发表时间：
2001-03-15
影响因子：
4.4
通讯作者：
Joly, E
中科院分区：
文献类型：
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作者：
Hudrisier, D;Riond, J;Joly, E
当CTL与携带外源Ag的靶细胞相遇时，TCR与其配体（肽-MHC I类复合物）内化。然而，目前还不清楚这是如何发生的机制，因为MHC分子通过跨膜结构域锚定到靶细胞的表面。通过使用荧光标记的抗原肽和脂质，我们发现CTL迅速捕获靶细胞膜连同抗原肽以及各种其他表面蛋白。这种有效和特异性的捕获过程需要持续的TCR信号传导。我们的观察结果表明，这个过程允许有效收购的Ag的CTL，这反过来可能会调节淋巴细胞的激活或消除。
Upon encounter of a CTL with a target cell carrying foreign Ags, the TCR internalizes with its ligand, the peptide-MHC class I complex. However, it is unclear how this can happen mechanistically because MHC molecules are anchored to the target cell's surface via a transmembrane domain. By using antigenic peptides and lipids that were fluorescently labeled, we found that CTLs promptly capture target cell membranes together with the antigenic peptide as well as various other surface proteins. This efficient and specific capture process requires sustained TCR signaling. Our observations indicate that this process allows efficient acquisition of the Ag by CTL, which may in turn regulate lymphocyte activation or elimination.