Harmine promotes periodontal ligament cell-induced tissue regeneration.
Harmine promotes periodontal ligament cell-induced tissue regeneration.
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DOI：
10.1111/odi.12770
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发表时间：
2018-04
期刊：
影响因子：
3.8
通讯作者：
H-C Lim;H-C Lim;Byung‐Yoon Cha;Sun U. Song;J. Yun
中科院分区：
文献类型：
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作者：
H-C Lim;H-C Lim;Byung‐Yoon Cha;Sun U. Song;J. Yun
目的探讨去氢骆驼蓬碱对人牙周膜细胞诱导的组织再生是否有促进作用。材料与方法检测不同浓度去氢骆驼蓬碱对人牙周膜细胞增殖的影响。通过茜素红S染色、实时荧光定量PCR和Western blotting检测hPDLC/rhBMP-2和hPDLC/去氢骆驼蓬碱的成骨和成牙骨质特性。在异位移植裸鼠模型中研究去氢骆驼蓬碱的活性。结果确定去氢骆驼蓬碱的阈值浓度为10 μM。与hPDLC/rhBMP-2相比，hPDLC/去氢骆驼蓬碱在茜素S染色中显示出类似的矿化结节形成。在实时PCR中，在所有时间点观察到Runx 2在hPDLC/去氢骆驼蓬碱中的最高基因表达水平。hPDLC/去氢骆驼蓬碱中CEMP-1的水平在第7天时高于单独的hPDLC。Western blotting显示，在第7天时，hPDLC/去氢骆驼蓬碱中Runx 2的条带比hPDLC/rhBMP-2中的要厚。在第7天和第14天，hPDLC/去氢骆驼蓬碱中CEMP-1的条带比单独的hPDLC更厚。在异位移植中，与rhBMP-2相比，含有去氢骆驼蓬碱的hPDLCs显示出相当量的矿化组织形成。hPDLCs与骆驼蓬碱或rhBMP-2形成骨和骨水泥样组织与Sharpey的纤维样胶原插入。结论去氢骆驼蓬碱可能是促进hPDLCs诱导的组织再生的潜在药物。
OBJECTIVE to investigate whether harmine has a promotive effect on human periodontal ligament cells (hPDLCs)-induced tissue regeneration. MATERIALS AND METHODS Various concentrations of harmine on hPDLCs proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC/rhBMP-2 and hPDLC/harmine by alizarin red S staining, real-time PCR, and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model. RESULTS We determined that 10 μM of harmine was the threshold concentration. hPDLC/harmine showed similar mineralized nodule formation in alizarin S staining compared to hPDLC/rhBMP-2. In real-time PCR, the highest gene expression level was observed for Runx2 in hPDLC/harmine at all time points. The level of CEMP-1 in hPDLC/harmine was higher at 7 days than hPDLCs alone. Thicker band of Runx2 in hPDLC/harmine was observed than in hPDLC/rhBMP-2 at 7 days by Western blotting. The band for CEMP-1 in hPDLC/harmine was thicker than hPDLCs alone at both 7 and 14 days. In ectopic transplantation, hPDLCs with harmine showed a comparable amount of mineralized tissue formation compared to rhBMP-2. hPDLCs with harmine or rhBMP-2 formed both bone and cementum-like tissue with Sharpey's fiber-like collagen insertion. CONCLUSION Harmine can be a potential candidate for promoting hPDLCs-induced tissue regeneration.