The Effects of Propofol in the Area Postrema of Rats
The Effects of Propofol in the Area Postrema of Rats
复制标题
异丙酚对大鼠后肢的影响
DOI：
10.1097/00000539-200104000-00027
复制
发表时间：
2001
影响因子：
5.7
通讯作者：
A. Gelb
中科院分区：
文献类型：
--
作者：
D. Cechetto;Tom Diab;C. J. Gibson;A. Gelb
丙泊酚具有止吐作用，其可能是由γ-氨基丁酸（GABA）对5-羟色胺系统的影响介导的，其机制尚不清楚。我们使用了三种技术，免疫组织化学，高效液相色谱法，和电生理学，以确定异丙酚对大鼠脑干的影响。成对的雄性Wistar大鼠接受丙泊酚（20 mg/kg/hr）或Intralid ®给药6 h。然后对大脑进行血清素的免疫组织化学分析。在一个单独的实验中，在输注丙泊酚或Intradolid ®后，从第四脑室提取脑脊液（CSF）并分析血清素和5-羟基吲哚乙酸的量。电生理神经元记录在最后区（AP）在异丙酚和GABA或5-羟色胺拮抗剂的反应。结果显示，与对照组相比，异丙酚组大鼠中缝背核5-羟色胺免疫组织化学染色显著增加（28 ± 12%），而AP的5-羟色胺免疫组织化学染色显著减少（17 ± 6%）。异氟烷麻醉动物中无显著变化。AP水平第四脑室CSF中的5-羟色胺和5-羟基吲哚乙酸分别显著降低63%和36%。异丙酚和戊巴比妥注射减少AP神经元的活动，但只有异丙酚的反应被荷包牡丹碱，GABA拮抗剂阻断。我们的结论是，在AP和CSF中的血清素水平的降低可以解释丙泊酚的止吐性质。丙泊酚也可以通过GABAA受体直接作用于AP神经元，以降低其活性。
Propofol has an antiemetic effect that may be mediated by &ggr;-aminobutyric acid (GABA) influences on the serotonin system, the mechanism of which is not known. We used three techniques, immunohistochemistry, High Performance Liquid Chromatography, and electrophysiology, to define propofol’s effects on the rat’s brainstem. Paired male Wistar rats received propofol, 20 mg/kg/hr, or Intralipid® for 6 h. The brains were then subjected to immunohistochemical analysis of serotonin. In a separate experiment after a propofol or Intralipid® infusion, cerebrospinal fluid (CSF) was extracted from the fourth ventricle and analyzed for the amount of serotonin and 5-hydroxyindoleacetic acid. Electrophysiological neuronal recordings were made in the area postrema (AP) in response to propofol with and without a GABA or serotonin antagonist. Results showed that immunohistochemical staining for serotonin in the propofol rats was significantly increased (28 ± 12%) in the dorsal raphe and decreased in the AP (17 ± 6%) compared with control. There were no significant changes in the isoflurane-anesthetized animals. Both serotonin and 5-hydroxyindoleacetic acid in the CSF of the fourth ventricle at the level of the AP were significantly reduced by 63% and 36%, respectively. Both propofol and pentobarbital injections reduce AP neuronal activity, but only the propofol response was blocked by bicuculline, a GABA antagonist. We conclude that the reduced levels of serotonin in the AP and the CSF may explain the antiemetic property of propofol. Propofol may also directly act on AP neurons via a GABAA receptor to reduce their activity.
DOI：
--
发表时间：
1984
期刊：
Federation proceedings
影响因子：
--
作者：
Pickel,VM;Armstrong,DM
通讯作者：
Armstrong,DM