Natural variation of DROT1 confers drought adaptation in upland rice.
Natural variation of DROT1 confers drought adaptation in upland rice.
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DOI：
10.1038/s41467-022-31844-w
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发表时间：
2022-07-23
影响因子：
16.6
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中科院分区：
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高地稻是一种生长在有氧环境中的独特生态类型，具有耐旱性。然而，其抗旱性的遗传基础尚不清楚。在这里，使用一个整合的方法结合全基因组关联研究与渐渗系和转录谱分析，我们确定了一个基因，DROUGHT 1（DROT 1），编码一个COBRA-like蛋白，赋予水稻抗旱性。DROT 1在维管束中特异性表达，并被ERF 3直接抑制，并被ERF 71激活，这两个干旱响应转录因子。DROT 1通过增加纤维素含量和保持纤维素结晶度来调节细胞壁结构，从而提高抗旱性。启动子中的C-to-T单核苷酸变异增加了高地中DROT 1的表达和抗旱性高地DROT 1潜在的优良单倍型可能来源于野生稻（O. rufipogon），并可能有利于高地品种的选育。高地耐旱性的遗传基础尚不清楚。在这里，作者报告了COBRA-like蛋白编码基因DROT 1的克隆，并揭示它被ERF 3抑制并被ERF 71激活，以帮助控制高地生长和耐旱性之间的平衡。
Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress. However, the genetic basis of its drought resistance is unclear. Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice. DROT1 is specifically expressed in vascular bundles and is directly repressed by ERF3 and activated by ERF71, both drought-responsive transcription factors. DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity. A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice. The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties. Genetic basis of the drought tolerance of upland rice is unclear. Here, the authors report the cloning of a COBRA-like protein encoding gene DROT1 and reveal that it is repressed by ERF3 and activated by ERF71 to help control the balance between growth and drought tolerance in upland rice.