Characterization of lecithin: cholesterol acyltransferase from human plasma: purification of the enzyme.
Characterization of lecithin: cholesterol acyltransferase from human plasma: purification of the enzyme.
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卵磷脂的表征：来自人血浆的胆固醇酰基转移酶：酶的纯化。
DOI：
10.1016/0003-9861(81)90437-9
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发表时间：
1981
影响因子：
3.9
通讯作者：
A. Lacko
中科院分区：
文献类型：
--
作者：
K. S. Chong;L. Davidson;R. G. Huttash;A. Lacko
卵磷脂：胆固醇酰基转移酶是用一种高效、高重复性的方法从人血浆中分离出来的。纯化方案包括：(1)疏水亲和层析，(2)deae -琼脂糖层析，(3)脱水，(4)第二次deae -琼脂糖层析，(5)羟基磷灰石和抗体吸附层析的组合。通过凝胶电泳、沉降平衡超离心、免疫扩散和免疫电泳，最终产品比起始材料纯化了2万倍，发现不含任何多肽污染物。
Lecithin:cholesterol acyltransferase was isolated from a human plasma fraction in a homogeneous state by an efficient and highly reproducible procedure. The purification scheme involved: (1) hydrophobic affinity chromatography, (2) DEAE-agarose chromatography, (3) delipidation, (4) a second DEAE-agarose chromatography, and (5) a combination of hydroxylapatite and antibody adsorption chromatography. The final product was purified 20,000-fold over the starting material and was found to be free of any polypeptide contaminant as indicated by gel electrophoresis, sedimentation equilibrium ultracentrifugation, immunodiffusion, and immunoelectrophoresis.