Biochemical and molecular effects of glycine betaine treatment on membrane fatty acid metabolism in cold stored peaches
Biochemical and molecular effects of glycine betaine treatment on membrane fatty acid metabolism in cold stored peaches
复制标题
甘氨酸甜菜碱处理对冷藏桃膜脂肪酸代谢的生化和分子影响
DOI：
10.1016/j.postharvbio.2019.04.007
复制
发表时间：
2019-08-01
影响因子：
7
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
--
作者：
Wang, Li;Bokhary, Syed Umar Farooq;Zheng, Yonghua
以桃为试材，采用生化和转录组学方法研究了甜菜碱（GB）处理对桃果实贮藏期间冷害、细胞膜完整性和膜脂脂肪酸代谢的影响。结果表明，GB处理通过抑制脂氧合酶（LOX）、磷脂酶D（PLD）和脂肪酶基因的表达和酶活性，提高不饱和脂肪酸含量和不饱和度，从而减轻CI。此外，本研究表明，GB处理可以抑制细胞色素C和caspase-3活性的释放，从而抑制细胞破裂，有助于膜功能。此外，转录组分析显示，与脂肪酸生物合成和去饱和相关的基因增强，而与脂肪酸降解和半胱天冬酶的激活相关的基因被GB处理抑制。因此，GB处理可通过上调膜脂脂肪酸合成和去饱和代谢，下调caspase活性，维持正常的细胞膜结构和功能，缓解桃CI。
The effect of glycine betaine (GB) treatment on chilling injury (CI), cell membrane integrity and membrane fatty acid metabolism in peach fruit during storage were investigated using biochemical and transcriptomic analysis in this study. The results showed that GB treatment alleviated the CI by suppressing lipoxygenase (LOX), phospholipase D (PLD) and lipase gene expression and enzyme activities, resulting in higher levels of unsaturated fatty acids and degree of unsaturation. In addition, this study suggested that GB treatment could inhibit the release of cytochrome C and caspase-3 activity, thus suppressing cell breakdown and contributing to membrane function. Moreover, transcriptome analysis revealed that genes related to fatty acid biosynthesis and desaturation were enhanced, while genes related to fatty acid degradation and activation of caspases were suppressed by GB treatment. Therefore, GB treatment could maintain normal cell membrane structure and function through upregulating membrane fatty acid biosynthesis and desaturation metabolism and downregulating caspase activation to alleviate CI in peaches.