Simultaneous determination of seven catechins in rat plasma by ultra-high performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetics study
Simultaneous determination of seven catechins in rat plasma by ultra-high performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetics study
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超高效液相色谱-串联质谱法同时测定大鼠血浆中7种儿茶素及其在药代动力学研究中的应用
DOI：
10.1039/c5ay01766a
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发表时间：
2015-11
影响因子：
3.1
通讯作者：
Wan, Xiaochun
中科院分区：
文献类型：
--
作者：
Xu, Liwei;Zhang, Shihua;Li, Junsong;Wan, Xiaochun
建立了一种快速、灵敏、高选择性的超高效液相色谱-串联质谱法测定大鼠血浆中7种儿茶素类化合物的方法。大鼠血浆用乙酸乙酯进行简单液-液萃取。采用Hypersil GOLD C18色谱柱（1.9 μm，50 × 2.1 mm），以甲醇-0.05%甲酸水溶液为流动相，采用梯度洗脱，以移动的为流动相，对血浆样品进行UHPLC分离。总运行时间为10.5 min，有效地分离了7种儿茶素。使用（-）-表儿茶素/（+）-儿茶素的相应跃迁m/z 289.070 → 109.010，对选择的反应监测进行检测，表没食子儿茶素/没食子儿茶素为305.089 → 125.228，表儿茶素没食子酸酯为441.150 → 169.325，表没食子儿茶素没食子酸酯为456.840 → 169.257- 没食子儿茶素没食子酸酯。7种儿茶素的平均回收率在84.92- 102.18%之间。日内和日间精密度（RSD）均小于6.17%和5.84%。该方法成功地应用于大鼠口服700 mg kg−1茶多酚后血浆中7种儿茶素的药代动力学研究。
A rapid, sensitive and selective ultra-high performance liquid chromatography tandem mass spectrometry method was developed and validated for the determination and pharmacokinetic investigation of seven catechins in rat plasma. The rat plasma was extracted with simple liquid–liquid extraction using ethyl acetate. Plasma sample was separated by UHPLC on a Hypersil GOLD C18 column (1.9 μm, 50 × 2.1 mm) using a mobile phase consisting of methanol–0.05% formic acid in water with gradient elution. The total run time was 10.5 min and seven catechins were efficiently separated. The detection was performed on a selected reaction monitoring using the respective transitions m/z 289.070 → 109.010 for (−)-epicatechin/(+)-catechin, 305.089 → 125.228 for (−)-epigallocatechin/(−)-gallocatechin and 441.150 → 169.325 for (−)-epicatechin gallate and 456.840 → 169.257 for (−)-epigallocatechin gallate/(−)-gallocatechin gallate. Mean recovery of seven catechins was in the range of 84.92–102.18%. The intra- and inter-day precisions (RSD) of these analytes were all less than 6.17% and 5.84%. This method was successfully applied in the pharmacokinetic study of seven catechins in the plasma of rats after oral administration of 700 mg kg−1 tea polyphenols.
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影响因子：
6.1
作者：
Ikeda, I;Kobayashi, M;Kakuda, T
通讯作者：
Kakuda, T
DOI：
--
发表时间：
1998-04
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Chung S. Yang;Laishun Chen;Mao-jung Lee;D. Balentine;May-Chen Kuo;S. Schantz
通讯作者：
Chung S. Yang;Laishun Chen;Mao-jung Lee;D. Balentine;May-Chen Kuo;S. Schantz
影响因子：
5.2
作者：
Forester, Sarah C.;Lambert, Joshua D.
通讯作者：
Lambert, Joshua D.
DOI：
--
发表时间：
1997
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
--
作者：
L. Chen;M. Lee;H. Li;C. S. Yang
通讯作者：
L. Chen;M. Lee;H. Li;C. S. Yang
DOI：
10.1016/j.jchromb.2014.07.028
发表时间：
2014-09-15
影响因子：
3
作者：
Wong, Ka Chun;Law, Man Chun;Chan, Tak Hang
通讯作者：
Chan, Tak Hang