Nanoparticle based DNA biosensor for tuberculosis detection using thermophilic helicase-dependent isothermal amplification
Nanoparticle based DNA biosensor for tuberculosis detection using thermophilic helicase-dependent isothermal amplification
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DOI：
10.1016/j.bios.2011.04.055
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发表时间：
2011-07-15
影响因子：
12.6
通讯作者：
Alocilja, Evangelyn C.
中科院分区：
文献类型：
--
作者：
Torres-Chavolla, Edith;Alocilja, Evangelyn C.
本研究描述了一种基于DNA的生物传感器的开发，以检测结核分枝杆菌使用嗜热解旋酶依赖的等温扩增（tHDA）和糊精包被的金纳米粒子（AuNPs）作为电化学报告。该生物传感器由金纳米颗粒（AuNPs）和胺封端的磁性颗粒（MP）组成，每个颗粒都用不同的DNA探针功能化，所述DNA探针与IS 16110基因内的片段的相对末端特异性杂交，所述IS 16110基因是M。结核复合体（MTC）特异性。杂交后，形成的复合物（MP-目标-AuNP）从溶液中磁性分离，并在丝网印刷碳电极（SPCE）芯片上电化学检测AuNP。获得的检测限为0.01 ng/μ l的等温扩增的目标（105 bp）。该生物传感器系统可以在外围实验室中使用便携式手持恒电位仪来实现。(C)2011 Elsevier B. V.保留所有权利。
The present study describes the development of a DNA based biosensor to detect Mycobacterium tuberculosis using thermophilic helicase-dependent isothermal amplification (tHDA) and dextrin coated gold nanoparticles (AuNPs) as electrochemical reporter. The biosensor is composed of gold nanoparticles (AuNPs) and amine-terminated magnetic particles (MPs) each functionalized with a different DNA probe that specifically hybridize with opposite ends of a fragment within the IS16110 gene, which is M. tuberculosis complex (MTC) specific. After hybridization, the formed complex (MP-target-AuNP) is magnetically separated from the solution and the AuNPs are electrochemically detected on a screen printed carbon electrode (SPCE) chip. The obtained detection limit is 0.01 ng/mu l of isothermally amplified target (105 bp). This biosensor system can be potentially implemented in peripheral laboratories with the use of a portable, handheld potentiostat. (C) 2011 Elsevier B.V. All rights reserved.