Integrating haplotypes and single genetic variability effects of the Pax7 gene on growth traits in two cattle breeds.
Integrating haplotypes and single genetic variability effects of the Pax7 gene on growth traits in two cattle breeds.
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DOI：
10.1139/gen-2012-0124
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发表时间：
2013
期刊：
影响因子：
3.1
通讯作者：
Yao Xu;Yang Zhou;Ning Wang;X. Lan;Chunlei Zhang;C. Lei;Hong Chen
中科院分区：
文献类型：
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作者：
Yao Xu;Yang Zhou;Ning Wang;X. Lan;Chunlei Zhang;C. Lei;Hong Chen
Pax 7基因编码的转录因子可以调控干细胞向肌源性细胞的转化，参与骨骼肌的发育和再生。本研究旨在通过DNA池测序和aCRS-RFLP方法检测5个品种1441头牛Pax 7基因的变异，并探讨其与南阳牛和中国草原红牛生长性状的相关性。总之，在Pax 7基因的最后一个内含子中鉴定了三个新的单核苷酸多态性（SNP）：NC_007300：ss 1（g. G103688A），ss2（g. T103735 C）和ss 3（g. A103764T）。5个品种间的基因型和参考单倍型频率具有较高的相似性，且3个基因座的G、T和A等位基因频率无论是单独还是组合都具有上级优势。单核苷酸多态性和单倍型组合的关联分析表明，ss 2和ss 3位点的T等位基因和单倍型H（2）H（2）（GG-TT-TT）对牛早期（6月龄和12月龄）的体高、体质量和胸围等生长性状有显著影响（P < 0.05）。结果表明，Pax 7基因变异及其对应的基因型可作为牛经济性状的分子标记。
The paired box 7 (Pax7) gene encoding for the transcription factor can regulate the conversion of stem cells into myogenic cells and participate in the development and regeneration of skeletal muscle. The aims of this study were to detect variations of the Pax7 gene by DNA pool sequencing and aCRS-RFLP methods in 1441 cattle from five breeds and to investigate their associations with growth traits in Nanyang and Chinese red steppe cattle. Altogether, three novel single nucleotide polymorphisms (SNPs) were identified in the last intron of the Pax7 gene: NC_007300: ss1 (g. G103688A), ss2 (g. T103735C), and ss3 (g. A103764T). Genotypes and the referring haplotype frequencies showed a high similarity trend among five breeds, and the G, T, and A allele frequencies of the three loci were always superior when separate or in combination. Association analysis of the single SNPs and haplotype combinations revealed that the T allele of ss2 and ss3 loci and the haplotype H(2)H(2) (GG-TT-TT) showed significant effects on growth traits such as body height, body mass, and chest girth in cattle at early stages (6 and 12 months old) (P < 0.05). The results showed that Pax7 gene variations and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.