Genetic fingerprinting of Theileria parva using a telomeric DNA probe
Genetic fingerprinting of Theileria parva using a telomeric DNA probe
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DOI：
10.1007/s004360050108
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发表时间：
1996-03-01
影响因子：
2
通讯作者：
Sohanpal, BK
中科院分区：
文献类型：
--
作者：
Bishop, RP;Spooner, PR;Sohanpal, BK
克隆的小泰勒虫端粒DNA序列命名为pTpUtel，用于表征T.通过与EcoRI消化的DNA杂交来获得parva原种和克隆。九个T中的八个。通过端粒限制性片段长度多态性（RFLP）来区分所测试的小球藻种群。用该探针对两个从布法罗7014中分离的蜱虫进行了鉴别。该探针在纯化的梨形虫或寄生虫感染的淋巴细胞DNA上得到了相当的结果，并且没有与未感染的牛DNA交叉杂交。克隆的T.寄生虫在蜱和牛体内传代4次后仍然保持相同。因此，端粒序列代表了一个有用的额外的工具，泰勒虫病流行病学分析。
A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLPs). Two isolates derived from buffalo 7014 by tick feeding on different occasions were also differentiated using the probe. The probe gave comparable results on purified piroplasm or schizont-infected lymphocyte DNA and did not cross-hybridize with uninfected bovine DNA. The telomeric restriction pattern of a cloned T. parva parasite remained identical after four passages through ticks and cattle. The telomeric sequence therefore represents a useful additional tool for analysis of theileriosis epidemiology.