Vulnerability of the human airway epithelium to hyperoxia. Constitutive expression of the catalase gene in human bronchial epithelial cells despite oxidant stress.
Vulnerability of the human airway epithelium to hyperoxia. Constitutive expression of the catalase gene in human bronchial epithelial cells despite oxidant stress.
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人类气道上皮对高氧的脆弱性。
DOI：
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发表时间：
1994
影响因子：
15.9
通讯作者：
R. Crystal
中科院分区：
文献类型：
--
作者：
J. Yoo;S. Erzurum;J. Hay;P. Lemarchand;R. Crystal
虽然过氧化氢酶是一种主要的细胞内抗氧化剂，人过氧化氢酶基因的表达似乎是有限的气道上皮细胞，使这些细胞容易受到氧化应激。这种有限的基因表达的基础进行了检查，通过评估的内源性基因在人支气管上皮细胞中的表达高氧。高氧未能上调内源性过氧化氢酶基因的表达，在热休克蛋白基因的表达显着增加。对人过氧化氢酶基因5 ′侧翼区1.7kb的序列分析显示了“管家”基因的特征（无TATA盒、高GC含量、多个CCAAT盒和转录起始位点）。用由不同长度的过氧化氢酶5 '侧翼区和荧光素酶作为报告基因组成的融合基因转染人支气管上皮细胞，显示出低水平的组成型启动子活性，其在暴露于高氧后没有改变。重要的是，使用含有人过氧化氢酶cDNA的复制缺陷型重组腺病毒载体，人气道上皮细胞中的过氧化氢酶水平显著增加，这与暴露于高氧时细胞存活率增加有关。这些观察结果为了解人气道上皮对氧化应激的敏感性和保护上皮免受这种损伤的策略提供了基础。
Although catalase is a major intracellular antioxidant, the expression of the human catalase gene appears to be limited in the airway epithelium, making these cells vulnerable to oxidant stress. The basis for this limited gene expression was examined by evaluation of the expression of the endogenous gene in human bronchial epithelial cells in response to hyperoxia. Hyperoxia failed to upregulate endogenous catalase gene expression, in contrast to a marked increase in expression of the heat shock protein gene. Sequence analysis of 1.7 kb of the 5'-flanking region of the human catalase gene showed features of a "house-keeping" gene (no TATA box, high GC content, multiple CCAAT boxes, and transcription start sites). Transfection of human bronchial epithelial cells with fusion genes composed of various lengths of the catalase 5'-flanking region and luciferase as a reporter gene showed low level constitutive promoter activity that did not change after exposure to hyperoxia. Importantly, using a replication-deficient recombinant adenoviral vector containing the human catalase cDNA, levels of catalase were significantly increased in human airway epithelial cells and this was associated with increased survival of the cells when exposed to hyperoxia. These observations provide a basis for understanding the sensitivity of the human airway epithelium to oxidant stress and a strategy for protecting the epithelium from such injury.
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影响因子：
--
作者：
F. Calzone;R. Britten;E. Davidson
通讯作者：
F. Calzone;R. Britten;E. Davidson
围产期大鼠肺过氧化氢酶基因表达：皮质类固醇和高氧的影响。
DOI：
10.1152/ajplung.1991.260.6.l428
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Clerch,LB;Iqbal,J;Massaro,D
通讯作者：
Massaro,D
影响因子：
10.4
作者：
Church DF;Pryor WA
通讯作者：
Pryor WA
DOI：
10.1016/s0021-9258(18)54242-3
发表时间：
1991-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Shull;Nicholas H. Heintz;Muthu Periasamy;M. Manohar;Yvonne W. M. Janssen;J. Marsh;Brooke T. Mossman-Brooke-T
通讯作者：
S. Shull;Nicholas H. Heintz;Muthu Periasamy;M. Manohar;Yvonne W. M. Janssen;J. Marsh;Brooke T. Mossman-Brooke-T
臭氧诱导大鼠肺抗氧化酶。
DOI：
10.1152/ajplung.1991.260.6.l412
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Rahman,I;Clerch,LB;Massaro,D
通讯作者：
Massaro,D