Formation of Protein Corona on Nanoparticles with Digestive Enzymes in Simulated Gastrointestinal Fluids
Formation of Protein Corona on Nanoparticles with Digestive Enzymes in Simulated Gastrointestinal Fluids
复制标题
在模拟胃肠液中消化酶纳米颗粒上形成蛋白冠
DOI：
10.1021/acs.jafc.8b05702
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发表时间：
2019-02-27
影响因子：
6.1
通讯作者：
Sun, Qingjie
中科院分区：
文献类型：
--
作者：
Wang, Yihui;Li, Man;Sun, Qingjie
蛋白质冠（PC），它定义了血液中纳米颗粒的生物学特性，是众所周知的，但尚未进行全面和系统的研究，在胃肠道环境中的PC的形成。因此，本研究旨在探索50-100 nm的模型聚苯乙烯纳米颗粒（PS-NPs）与三种消化酶，即胃蛋白酶，α-淀粉酶和胰蛋白酶之间的相互作用。结果表明，α-淀粉酶和胰蛋白酶形成的PC的厚度分别为25-100和50-100 nm。温育后PS-NP的zeta-电位值显著增加。荧光猝灭和紫外-可见吸收光谱表明纳米颗粒与酶之间发生了相互作用。同步荧光光谱表明，PS-NPs可引起消化酶微环境的变化。热力学参数表明，相互作用主要由氢键和货车范德华力驱动。
The protein corona (PC), which defines the biological identity of nanoparticles in the blood, is well-known, but no comprehensive and systematic study has been conducted yet on the formation of PCs in the gastrointestinal environment. Thus, this study aimed to explore the interaction between model polystyrene nanoparticles (PS-NPs) of 50-100 nm and three digestive enzymes, namely, pepsin, alpha-amylase, and trypsin. Results showed that the thicknesses of the PCs formed by alpha-amylase and trypsin were 25-100 and 50-100 nm, respectively. The zeta-potential values of PS-NPs after incubation significantly increased. The fluorescence quenching and ultraviolet-visible-absorption spectra suggested that interactions between the nanoparticles and the enzymes occurred. Synchronous fluorescence spectra showed that the PS-NPs could induce microenvironmental changes in digestive enzymes. The thermodynamic parameters suggested that the interaction was mainly driven by hydrogen bonds and van der Waals forces.