Layered gelatin/PLLA scaffolds fabricated by electrospinning and 3D printing- for nasal cartilages and subchondral bone reconstruction
Layered gelatin/PLLA scaffolds fabricated by electrospinning and 3D printing- for nasal cartilages and subchondral bone reconstruction
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DOI：
10.1016/j.matdes.2018.06.012
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发表时间：
2018-10-05
影响因子：
8.4
通讯作者：
Menaszek, Elzbieta
中科院分区：
文献类型：
--
作者：
Rajzer, Izabella;Kurowska, Anna;Menaszek, Elzbieta
在目前的工作中，结合了两种不同的、众所周知的和适用的生物材料（PLLA和明胶）和两种支架制造技术（3D打印-FDM和静电纺丝）的优点，以创建一种用于鼻软骨和软骨下骨重建的新型多功能分层支架。利用3D打印技术制作的支架孔径，旨在解决目前耳鼻喉科医生用针线固定鼻软骨植入物的难题。研究了静电纺丝过程中溶液浓度对明胶纳米纤维微观结构和机械性能的影响以及药物浓度对膜机械性能的影响。使用市售的FDM-3D打印系统。设计并打印了结构和几何形状各异的 3D 支架。测试了 3D 打印支架的内部结构对其机械性能的影响。开发了由顶部明胶纳米纤维层和底部 3D 打印多孔 PLLA 材料组成的混合层状支架。在模拟体液中测定支架的矿化能力。评估了在获得的生物材料上培养的鼠成纤维细胞L929的细胞毒性、增殖和形态。 (C) 2018 Elsevier Ltd. 保留所有权利。
In the present work the advantages of two kinds of different, well known and applicable, biomaterials (PLLA and gelatin) and twokinds of scaffold fabrication techniques (3D printing - FDMand electrospinning) were combined in order to create a novel multifunctional layered scaffold for nasal cartilages and subchondral bone reconstruction. The pore size of scaffolds produced by 3D printing technology was designed to solve the problem that oto-laryngologists currently have with fixing the nasal cartilage implant with needle and threads. The effect of the solution concentration for the electrospinning process on the microstructure and mechanical properties of gelatin nanofibers produced as well as the influence of drug concentration on the mechanical properties of membranes were investigated. The commercially available FDM - 3D printing system was used. 3D scaffolds varying in structure and geometry were designed and printed. The influence of the internal architecture of 3D printed scaffolds on their mechanical properties was tested. Hybrid layered scaffolds consisting of a top gelatin nanofibrous layer and a bottom 3D printed porous PLLA material were developed. The mineralization ability of a scaffold was determined in simulated body fluid. The cytotoxicity, proliferation and morphology of Murine fibroblasts L929 cultured on obtained biomaterials were evaluated. (C) 2018 Elsevier Ltd. All rights reserved.