Increases in cytochrome p-450 in cultured hepatocytes mediated by 3- and 4-carbon alcohols.
Increases in cytochrome p-450 in cultured hepatocytes mediated by 3- and 4-carbon alcohols.
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由 3 碳和 4 碳醇介导的培养肝细胞中细胞色素 p-450 的增加。
DOI：
10.1016/0006-2952(82)90138-1
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发表时间：
1982
影响因子：
5.8
通讯作者：
Bonkowsky,HL
中科院分区：
文献类型：
--
作者：
Sinclair,JF;Smith,L;Bement,WJ;Sinclair,PR;Bonkowsky,HL
用正丙醇、异丙醇、正丁醇或异丁醇处理培养的鸡胚肝细胞后，细胞色素P-450的量有不同程度的增加，这些增加与苯丙胺脱甲基酶活性的增加、细胞色素P-450催化的氧化以及UDP-葡萄糖醛酸基转移酶催化的酚红的葡萄糖醛酸化有关。该反应与使用乙醇或丙基异丙基乙酰胺（苯巴比妥样诱导剂）获得的反应相似。用放线菌酮预处理细胞可防止细胞色素 P-450 和酚红葡萄糖醛酸化的增加，表明这些反应需要蛋白质合成。
The amount of cytochrome P-450 was increased to different extents after treatment of cultured chick embryo hepatocytes withn-propanol, isopropanol,n-butanol, or isobutanol, These increases were associated with increases in benzphetamine demethylase activity, a cytochrome P-450-catalyzed oxidation, and glucuronidation of phenol red, catalyzed by UDP-glucuronyl transferase. The responses were similar to those obtained with ethanol or propylisopropylacetamide, which are phenobarbital-like inducers. Pretreatment of cells with cycloheximide prevented the increases in both cytochrome P-450 and glucuronidation of phenol red, indicating that protein synthesis was required for these responses.