Chromosome Location, Linkage with Simple Sequence Repeat Markers, and Leaf Rust Resistance Conditioned by Gene Lr63 in Wheat
Chromosome Location, Linkage with Simple Sequence Repeat Markers, and Leaf Rust Resistance Conditioned by Gene Lr63 in Wheat
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DOI：
10.2135/cropsci2010.01.0005
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发表时间：
2010-11-01
期刊：
影响因子：
2.3
通讯作者：
Flor, J. M.
中科院分区：
文献类型：
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作者：
Kolmer, J. A.;Anderson, J. A.;Flor, J. M.
小麦（Triticum aestivum L.）撒切尔（Thatcher）回交系RL6137对单球菌（T monococum）衍生的小麦叶锈病病原小麦锈病（Puccinia triticina）具有抗性。为了确定该抗性的染色体定位，将RL6137与Thatcher杂交，并对F(2)株苗进行了叶锈病抗性分离和SSR标记分离试验。利用F(3)家族分离鉴定了F(2)个体的抗叶锈病基因型。F(2)幼苗和F(3)家族因单一抗叶锈病基因而分离。位于3AS染色体上的SSR标记barc57和barc321与抗叶锈病基因紧密连锁。将RL6137的抗叶锈病基因定为Lr63。该基因对大多数小麦短叶锈病分离株具有低至中等感染类型，可用于小麦改良计划，以提高小麦的抗叶锈病能力。
RL6137, a 'Thatcher' backcross line of wheat (Triticum aestivum L.) has resistance to Puccinia triticina (causal agent of wheat leaf rust) derived from T monococcum. To determine the chromosome location of this resistance, RL6137 was crossed with Thatcher and F(2) individual seedlings were tested for segregation of leaf rust resistance and segregation of polymorphic simple sequence repeat (SSR) markers. Leaf rust resistance genotypes of F(2) individuals were confirmed with segregation of F(3) families. The F(2) seedlings and F(3) families segregated for a single leaf rust resistance gene. The SSR markers barc 57 and barc 321 located on chromosome 3AS were tightly linked with the leaf rust resistance gene. The leaf rust resistance gene in RL6137 was designated as Lr63. This gene conditions low to intermediate infection types to most P. triticina isolates and can be used in wheat improvement programs to enhance leaf rust resistance.