The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells
The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells
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DOI：
10.1016/j.virol.2011.12.001
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发表时间：
2012-02-20
期刊：
影响因子：
3.7
通讯作者：
Morris, Lynn
中科院分区：
文献类型：
--
作者：
Alexandre, Kabamba B.;Gray, Elin S.;Morris, Lynn
一般认为，在HIV-1的性传播过程中，聚糖特异性DC-SIGN受体结合病毒并介导其转移到CD 4（+）细胞。凝集素griffithsin（GRFT）、cyanovirin-N（CV-N）和scytovirin（SVN）通过与gp 120上富含甘露糖的聚糖结合来抑制HIV-1感染。我们测量了这些凝集素抑制HIV-1与DC-SIGN结合以及DC-SIGN介导的HIV-1感染CD 4（+）细胞的能力。虽然GRFT、CV-N和SVN对DC-SIGN结合具有中度抑制作用，但它们有效抑制HIV-1的DC-SIGN转移。234糖基化位点的引入消除了HIV-1对凝集素抑制结合DC-SIGN和病毒转移至易感细胞的敏感性。然而，295糖基化位点的添加增加了转移的抑制。我们的数据表明，GRFT。CV-N和SVN可以阻断HIV-1性传播的两个重要阶段，DC-SIGN结合和转移，支持它们作为杀微生物剂的进一步发展。(C)2011 Elsevier Inc. All rights reserved.
It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) inhibit HIV-1 infection by binding to mannose-rich glycans on gp120. We measured the ability of these lectins to inhibit both the HIV-1 binding to DC-SIGN and the DC-SIGN-mediated HIV-1 infection of CD4(+) cells. While GRFT, CV-N and SVN were moderately inhibitory to DC-SIGN binding, they potently inhibited DC-SIGN-transfer of HIV-1. The introduction of the 234 glycosylation site abolished HIV-1 sensitivity to lectin inhibition of binding to DC-SIGN and virus transfer to susceptible cells. However, the addition of the 295 glycosylation site increased the inhibition of transfer. Our data suggest that GRFT. CV-N and SVN can block two important stages of the sexual transmission of HIV-1, DC-SIGN binding and transfer, supporting their further development as microbicides. (C) 2011 Elsevier Inc. All rights reserved.