Expression of Bmi-1 is a prognostic marker in bladder cancer.
Expression of Bmi-1 is a prognostic marker in bladder cancer.
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Bmi-1的表达是膀胱癌的预后标志物
DOI：
10.1186/1471-2407-9-61
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发表时间：
2009-02-19
期刊：
影响因子：
3.8
通讯作者：
Zeng MS
中科院分区：
文献类型：
--
作者：
Qin ZK;Yang JA;Ye YL;Zhang X;Xu LH;Zhou FJ;Han H;Liu ZW;Song LB;Zeng MS
背景膀胱癌发生、发展的分子机制尚不清楚.本研究旨在探讨Bmi-1蛋白在膀胱癌组织中的表达及其临床意义。方法采用RT-PCR和Western blot方法分别检测14例膀胱癌组织及癌旁正常组织中Bmi-1 mRNA和Bmi-1蛋白的表达。采用免疫组化法检测137例膀胱癌组织和30例癌旁正常膀胱组织中Bmi-1蛋白的表达。结果14例膀胱癌组织中Bmi-1 mRNA和蛋白的表达均高于癌旁正常组织（P <0.01），而Bmi-1 mRNA和蛋白的表达均低于癌旁正常组织（P< 0.01）。Bmi-1蛋白在30例癌旁正常膀胱组织中阳性表达率为16.7%，在137例膀胱癌组织中阳性表达率为54.3%（P< 0.05）。Bmi-1蛋白在G1、G2和G3期的阳性表达率分别为20.6%、54.3%和78.8%（P< 0.05）。浸润性膀胱癌中Bmi-1蛋白的表达明显高于表浅性膀胱癌（81.5%比32.5%，P < 0.05）。在浸润性膀胱癌中，Bmi-1蛋白在无进展膀胱癌和有进展膀胱癌中的表达率相似（80.0%vs82.4%，P> 0.5）。浅表性膀胱癌中Bmi-1蛋白的表达在复发组明显高于无复发组（62.5%比13.7%，P < 0.05）。Bmi-1的表达与肿瘤的分型、TNM分期呈正相关（P< 0.05），而与肿瘤的个数无关（P> 0.05）。Bmi-1高表达组5年生存率为50.8%，低表达组为78.5%（P< 0.05）。结论膀胱癌组织中Bmi-1的表达高于癌旁正常组织，Bmi-1的表达与膀胱癌的发生发展密切相关。检测Bmi-1蛋白表达对膀胱癌的预后评估具有潜在价值。
BackgroundThe molecular mechanisms of the development and progression of bladder cancer are poorly understood. The objective of this study was to analyze the expression of Bmi-1 protein and its clinical significance in human bladder cancer.MethodsWe examined the expression of Bmi-1 mRNA and Bmi-1 protein by RT-PCR and Western blot, respectively in 14 paired bladder cancers and the adjacent normal tissues. The expression of Bmi-1 protein in 137 specimens of bladder cancer and 30 specimens of adjacent normal bladder tissue was determined by immunohistochemistry. Statistical analyses were applied to test the relationship between expression of Bmi-1, and clinicopathologic features and prognosis.ResultsExpression of Bmi-1 mRNA and protein was higher in bladder cancers than in the adjacent normal tissues in 14 paired samples (P< 0.01). By immunohistochemical examination, five of 30 adjacent normal bladder specimens (16.7%) versus 75 of 137 bladder cancers (54.3%) showed Bmi-1 protein expression (P< 0.05). Bmi-1 protein expression was intense in 20.6%, 54.3%, and 78.8% of tumors of histopathological stages G1, G2, and G3, respectively (P< 0.05). Expression of Bmi-1 protein was greater in invasive bladder cancers than in superficial bladder cancers (81.5% versus 32.5%,P< 0.05). In invasive bladder cancers, the expression of Bmi-1 protein in progression-free cancers was similar to that of cancers that have progressed (80.0% versus 82.4%,P> 0.5). In superficial bladder cancers, the expression of Bmi-1 protein in recurrent cases was higher than in recurrence-free cases (62.5% versus 13.7%,P< 0.05). Bmi-1 expression was positively correlated with tumor classification and TNM stage (P< 0.05), but not with tumor number (P> 0.05). Five-year survival in the group with higher Bmi-1 expression was 50.8%, while it was 78.5% in the group with lower Bmi-1 expression (P< 0.05). Patients with higher Bmi-1 expression had shorter survival time, whereas patients with lower Bmi-1 expression had longer survival time (P< 0.05).ConclusionExpression of Bmi-1 was greater in bladder cancers than in the adjacent normal tissues. The examination of Bmi-1 protein expression is potentially valuable in prognostic evaluation of bladder cancer.
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影响因子：
5.3
作者：
Mori, T;Kiyono, T;Umezawa, A
通讯作者：
Umezawa, A
Bmi-1 对于神经母细胞瘤细胞的致瘤性至关重要
影响因子：
6
作者：
Cui, Hongjuan;Hu, Bo;Ding, Han-Fei
通讯作者：
Ding, Han-Fei
影响因子：
6.2
作者：
Saito, M;Handa, K;Teranaka, T
通讯作者：
Teranaka, T
影响因子：
7.8
作者：
Ball, AJ;Levine, F
通讯作者：
Levine, F
影响因子：
6.6
作者：
Chang, LL;Yeh, WT;Huang, CH
通讯作者：
Huang, CH