The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex
The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex
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DOI：
10.1093/emboj/17.14.4086
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发表时间：
1998-07-15
期刊：
影响因子：
11.4
通讯作者：
Reece, RJ
中科院分区：
文献类型：
--
作者：
Platt, A;Reece, RJ
酿酒酵母通过激活编码Leloir途径的酶的GAL基因来响应作为唯一碳源的半乳糖。在这里，我们表明，在体外的开关从压抑到激活的基因表达涉及三种蛋白质的相互作用[激活剂（Gal 4p），阻遏物（Gal 80 p）和诱导剂（Gal 3 p）]和两个小分子（半乳糖和ATP）。我们还表明，半乳糖和ATP依赖性的相互作用之间的Gal 3 p和Gal 80 p发生没有中断的Gal 80 p-Gal 4p的相互作用。因此，Gal 3 p介导的转录激活通过形成三重蛋白复合物而发生。
Saccharomyces cerevisiae responds to galactose as the sole source of carbon by activating the GAL genes encoding the enzymes of the Leloir pathway. Here, we show in vitro that the switch from repressed to activated gene expression involves the interplay of three proteins [an activator (Gal4p), a repressor (Gal80p) and an inducer (Gal3p)] and two small molecules (galactose and ATP). We also show that the galactose- and ATP-dependent interaction between Gal3p and Gal80p occurs without disruption of the Gal80p-Gal4p interaction. Thus, Gal3p-mediated activation of transcription occurs via the formation of a tripartite protein complex.