Adaptive optics scanning laser ophthalmoscope with integrated wide-field retinal imaging and tracking.
Adaptive optics scanning laser ophthalmoscope with integrated wide-field retinal imaging and tracking.
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DOI：
10.1364/josaa.27.00a265
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发表时间：
2010-11-01
期刊：
影响因子：
--
通讯作者：
Burns SA
中科院分区：
文献类型：
--
作者：
Ferguson RD;Zhong Z;Hammer DX;Mujat M;Patel AH;Deng C;Zou W;Burns SA
我们已经开发出一种新的，统一的自适应光学扫描激光检眼镜（AOSLO）的实施纳入宽场线扫描检眼镜（LSO）和闭环光学视网膜跟踪器。AOSLO光栅扫描由集成跟踪镜偏转，因此在跟踪过程中自动实现直接AOSLO稳定。宽视场成像器和大球面镜光学接口设计，以及大行程变形镜（DM），使AOSLO像场能够在大于25度的视场中，在任何感兴趣的视网膜坐标处进行校正。通过对具有一系列屈光不正的个体进行成像来评估AO性能。在大多数受试者中，图像对比度在接近衍射极限的空间频率下是可测量的。闭环光学（硬件）跟踪性能进行了评估，通过比较序列图像系列与不稳定。虽然通常优于10 μm rms或0.03 deg，但跟踪尚未稳定到单锥精度，但可显著提高平均图像质量，并增加可通过基于软件的后处理方法成功对齐的帧数。新的光学接口允许将高分辨率成像场放置在宽视场内的任何位置，而无需受试者重新固定，从而实现更轻松的视网膜导航和更快、更高效的AOSLO蒙太奇捕获和拼接。
We have developed a new, unified implementation of the adaptive optics scanning laser ophthalmoscope (AOSLO) incorporating a wide-field line-scanning ophthalmoscope (LSO) and a closed-loop optical retinal tracker. AOSLO raster scans are deflected by the integrated tracking mirrors so that direct AOSLO stabilization is automatic during tracking. The wide-field imager and large-spherical-mirror optical interface design, as well as a large-stroke deformable mirror (DM), enable the AOSLO image field to be corrected at any retinal coordinates of interest in a field of >25 deg. AO performance was assessed by imaging individuals with a range of refractive errors. In most subjects, image contrast was measurable at spatial frequencies close to the diffraction limit. Closed-loop optical (hardware) tracking performance was assessed by comparing sequential image series with and without stabilization. Though usually better than 10 μm rms, or 0.03 deg, tracking does not yet stabilize to single cone precision but significantly improves average image quality and increases the number of frames that can be successfully aligned by software-based post-processing methods. The new optical interface allows the high-resolution imaging field to be placed anywhere within the wide field without requiring the subject to re-fixate, enabling easier retinal navigation and faster, more efficient AOSLO montage capture and stitching.