Genomic analysis of two ORF2 segments of new porcine astrovirus isolates and their close relationship with human astroviruses
Genomic analysis of two ORF2 segments of new porcine astrovirus isolates and their close relationship with human astroviruses
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DOI：
10.1139/w10-042
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发表时间：
2010-07-01
影响因子：
2.8
通讯作者：
Fernanda Gutierrez, Maria
中科院分区：
文献类型：
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作者：
Carlos Ulloa, Juan;Fernanda Gutierrez, Maria
猪星状病毒（PAstV）的研究很少，主要与胃肠炎有关。计算分析揭示了PAstV与人星状病毒（HAstV）和猫星状病毒（FAstV）的密切关系。在这项研究中，105和171粪便标本收集了小猪和5岁以下的儿童，分别在不同的哥伦比亚地区在1年的时间。用ELISA和RTPCR检测粪便样品中的星状病毒，PAstV和HAstV阳性率分别为23.8%和4%。对从ORF2获得的部分序列的额外序列分析鉴定了至少2个可能的PAstV组和猪与人星状病毒之间可能的重组事件。这项研究提供了初步证据，在猪中的高存在的PAstV，并提出了存在多种PAstV类型的进化密切相关的HAstV。
Porcine astrovirus (PAstV) has been poorly studied and has been associated mainly with gastroenteritis. Computational analysis has revealed the close relationship of PAstV with astroviruses of humans (HAstV) and cats (FAstV). In this study, 105 and 171 stool specimens were collected from piglets and children under 5 years of age, respectively, in different Colombian regions during a 1-year period. The stool samples were examined for astroviruses by ELISA and RTPCR; 23.8% and 4% were found to be positive for PAstV and HAstV, respectively. Additional sequence analysis with partial sequences obtained from ORF2 identified at least 2 probable groups of PAstVs and possible recombination events between porcine and human astroviruses. This study provides preliminary evidence of the high presence of PAstVs in pigs and proposes the existence of multiple PAstV types that are evolutionarily closely related to HAstVs.