Functional evidence for indirect recognition of G center dot U in tRNA(Ala) by alanyl-tRNA synthetase
Functional evidence for indirect recognition of G center dot U in tRNA(Ala) by alanyl-tRNA synthetase
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DOI：
10.1126/science.271.5246.195
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发表时间：
1996-01-12
期刊：
影响因子：
56.9
通讯作者：
McClain, WH
中科院分区：
文献类型：
--
作者：
Gabriel, K;Schneider, J;McClain, WH
G的结构特点大肠杆菌丙氨酸转移 RNA (tRNA(Ala)) 中与丙氨酰-tRNA 合成酶 (AlaRS) 氨酰化相关的 U 摆动对在体内研究了野生型 tRNA(Ala) 和带有 G 的突变 tRNA。 U 换人。 tRNA(Ala) 与 G 。乌，C。 A 或 G 。 A 给出相似量的带电 tRNA(Ala)，并支持缺乏染色体 tRNA(Ala) 基因的大肠杆菌的活力。 tRNA(Ala) 与 G 。 C 不活动。 G 的认可。因此，AlaRS 的 U 需要的不仅仅是 G 上的官能团。 U 呈规则螺旋，并且可能涉及螺旋变形的检测。
The structural features of the G . U wobble pair in Escherichia coli alanine transfer RNA (tRNA(Ala)) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNA(Ala) and mutant tRNAs with G . U substitutions. tRNA(Ala) with G . U, C . A, or G . A gave similar amounts of charged tRNA(Ala) and supported viability of E. coli lacking chromosomal tRNA(Ala) genes. tRNA(Ala) With G . C was inactive. Recognition of G . U by AlaRS thus requires more than the functional groups on G . U in a regular helix and may involve detection of a helical distortion.