Effects of insulin-like growth factor-I and its analogues on bovine hydrogen peroxide release by neutrophils and blastogenesis by mononuclear cells.
Effects of insulin-like growth factor-I and its analogues on bovine hydrogen peroxide release by neutrophils and blastogenesis by mononuclear cells.
复制标题
胰岛素样生长因子-I 及其类似物对牛中性粒细胞过氧化氢释放和单核细胞胚泡发生的影响。
DOI：
10.1677/joe.0.1390259
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. H. Burton
中科院分区：
文献类型：
--
作者：
X. Zhao;B. McBride;L. Trouten;J. H. Burton
重组人胰岛素样生长因子-I（IGF-I）和它的两个类似物的生物学效力进行了检查过氧化氢释放的中性粒细胞和单核细胞的胚细胞。还研究了这些肽对牛血清IGF结合蛋白（IGFBPs）和牛中性粒细胞和单核细胞上的IGF-I受体的结合亲和力。相对于不含IGF-I的对照处理，用12.5 μ g/l IGF-I预孵育中性粒细胞，des（1-3）IGF-I（缺少N-末端三肽Gly-Pro-Glu的人IGF-I的类似物）和长R3 IGF-I（人IGF-I的类似物，其中精氨酸取代人IGF-I第3位的谷氨酸，N末端延伸Met-Phe-Pro-Ala-Met-Pro-Leu-Ser-Ser-Leu-Phe-Val-Asn）H_2O_2释放量分别增加65%、64%和32%。然而，在100微克/升的剂量下，长R3 IGF-I和其他两种（IGF-I和des（1-3）IGF-I）之间刺激H2 O2释放的差异减小。在不存在或存在2.5%胎牛血清（FCS）的情况下，100 μ g/l的IGF-I，des（1-3）IGF-I而不是长R3 IGF-I显著刺激胸苷掺入单核细胞。此外，在2.5%FCS存在下，des（1-3）IGF-I比IGF-I更有效地刺激胸苷掺入单核细胞。（250字处删节）
The biological potencies of recombinant human insulin-like growth factor-I (IGF-I) and two of its analogues were examined for hydrogen peroxide release by neutrophils and blastogenesis by mononuclear cells. The binding affinities of these peptides for bovine serum IGF-binding proteins (IGFBPs) and IGF-I receptors on bovine neutrophils and mononuclear cells were also investigated. Relative to control treatment containing no IGF-I, preincubation of neutrophils with 12.5 micrograms/l of IGF-I, des(1-3)IGF-I (an analogue of human IGF-I lacking the N-terminal tripeptide Gly-Pro-Glu) and long R3 IGF-I (an analogue of human IGF-I with arginine replacing glutamate at position 3 of human IGF-I and the N-terminal extension Met-Phe-Pro-Ala-Met-Pro-Leu-Ser-Ser-Leu-Phe-Val-Asn) increased the release of H2O2 by 65%, 64% and 32% respectively. However, the difference in stimulating the release of H2O2 between long R3 IGF-I and other two (IGF-I and des(1-3)IGF-I) was reduced at a dosage of 100 micrograms/l. In the absence or presence of 2.5% fetal calf serum (FCS), 100 micrograms/l of IGF-I, des(1-3)IGF-I but not long R3 IGF-I significantly stimulated thymidine incorporation into mononuclear cells. In addition, des(1-3)IGF-I was more potent than IGF-I in stimulating thymidine incorporation into mononuclear cells in the presence of 2.5% FCS.(ABSTRACT TRUNCATED AT 250 WORDS)