Expression of a Single-Copy hsp-16.2 Reporter Predicts Life span
Expression of a Single-Copy hsp-16.2 Reporter Predicts Life span
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DOI：
10.1093/gerona/glr225
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发表时间：
2012-07-01
影响因子：
5.1
通讯作者：
Johnson, Thomas E.
中科院分区：
文献类型：
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作者：
Mendenhall, Alexander R.;Tedesco, Patricia M.;Johnson, Thomas E.
基于hsp-16.2的转录报告子的绿色荧光蛋白表达水平预测秀丽隐杆线虫的寿命和耐热性。最初的报告使用了整合到IV号染色体中的高拷贝数报告基因。有人担心，这位报告者的寿命预测能力不仅仅归因于hsp-16.2的输出。具体来说，预测能力可能来自于gpIs 1插入对IV号染色体上某些关键染色质的破坏，这是一种用于创建报告基因的过程中的连锁突变，或者来自转基因调控的人为因素（多拷贝转基因受到秀丽隐杆线虫染色质监视机制的调控）。在这里，我们确定是否有能力预测寿命和耐热性是特定的gpIs 1插入或一般属性的HSP-16.2为基础的报告。新的单拷贝hsp-16.2报告基因预测寿命和耐热性。我们的结论是，预测能力的热休克蛋白-16.2为基础的转录报告是不是任何特定的转基因配置或染色质监视机制的文物。
The level of green fluorescent protein expression from an hsp-16.2-based transcriptional reporter predicts life span and thermotolerance in Caenorhabditis elegans. The initial report used a high-copy number reporter integrated into chromosome IV. There was concern that the life-span prediction power of this reporter was not attributable solely to hsp-16.2 output. Specifically, prediction power could stem from disruption of some critical piece of chromatin on chromosome IV by the gpIs1 insertion, a linked mutation from the process used to create the reporter, or from an artifact of transgene regulation (multicopy transgenes are subject to regulation by C elegans chromatin surveillance machinery). Here we determine if the ability to predict life span and thermotolerance is specific to the gpIs1 insertion or a general property of hsp-16.2-based reporters. New single-copy hsp-16.2-based reporters predict life span and thermotolerance. We conclude that prediction power of hsp-16.2-based transcriptional reporters is not an artifact of any specific transgene configuration or chromatin surveillance mechanism.