Monocyte differentiation in localized juvenile periodontitis is skewed toward the dendritic cell phenotype
Monocyte differentiation in localized juvenile periodontitis is skewed toward the dendritic cell phenotype
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DOI：
10.1128/iai.70.6.2780-2786.2002
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发表时间：
2002-06-01
影响因子：
3.1
通讯作者：
Tew, JG
中科院分区：
文献类型：
--
作者：
Barbour, SE;Ishihara, Y;Tew, JG
多项证据表明，患有局限性青少年牙周炎（LJP）受试者的单核细胞在功能上与年龄和种族匹配的非牙周炎（NP）受试者的细胞不同。异常包括分泌大量前列腺素 E-2 的倾向和免疫球蛋白 G2 (IgG2) 抗体的诱导。进行此处描述的实验是为了进一步表征 LJP 单核细胞并确定这些细胞的成熟是否与 NP 单核细胞不同。当来自 LJP 受试者的贴壁单核细胞在人血清存在下培养时，观察到巨噬细胞和具有未成熟单核细胞衍生树突状细胞 (MDDC) 形态的细胞。 4 天内，LJP 培养物中未成熟 MDDC 的患病率大约是 NP 培养物中的两倍。除了树突形态外，这些细胞还具有 CD11c(+) 和 CD14(-) 或 CD14(低) 特征，并刺激有效的自体混合白细胞反应，与向 MDDC 表型的分化一致。与 UP 单核细胞一样，用白细胞介素 4 和粒细胞巨噬细胞集落刺激因子生成的 MDDC 培养物在商陆丝裂原刺激的 NP 白细胞培养物中选择性诱导 IgG2。总之，这些数据表明 LJP 受试者的单核细胞有分化为 MDDC 的倾向，并且这种分化可能与 UP 受试者血清中观察到的高水平 IgG2 有关。由于高水平的循环 IgG2 与较轻的疾病相关，LJP 单核细胞分化为 MDDC 的倾向可能对宿主针对口腔病原体的反应和 UP 的进展具有重要影响。
Several lines of evidence indicate that the monocytes of subjects with localized juvenile periodontitis (LJP) are functionally distinct from cells of age- and race-matched nonperiodontitis (NP) subjects. Among the abnormalities are the propensity to secrete large amounts of prostaglandin E-2 and the induction of immunoglobulin G2 (IgG2) antibodies. The experiments described here were performed to further characterize the LJP monocytes and to determine if these cells mature differently than NP monocytes. When adherent monocytes from LJP subjects were cultured in the presence of human serum, both macrophages and cells with the morphology of immature monocyte-derived dendritic cells (MDDC) were observed. Within 4 days the prevalence of the immature MDDC was approximately twofold higher in LJP cultures than in NP cultures. In addition to their dendritic morphology, these cells were CD11c(+) and CD14(-) or CD14(low) and stimulated potent autologous mixed leukocyte reactions, consistent with differentiation to the MDDC phenotype. Like UP monocytes, cultures of MDDC generated with interleukin-4 and granulocyte-macrophage colony-stimulating factor selectively induced IgG2 in cultures of pokeweed mitogen-stimulated NP leukocytes. Together, these data suggest that the monocytes of LJP subjects have a propensity to differentiate into MDDC and that this differentiation may be related to the high levels of IgG2 that are observed in the sera of UP subjects. As high levels of circulating IgG2 are correlated with less severe disease, the propensity of LJP monocytes to differentiate into MDDC may have important implications for both the host response against oral pathogens and the progression of UP.