Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens
Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens
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DOI：
10.1007/s11104-020-04512-5
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发表时间：
2020-05-06
期刊：
影响因子：
4.9
通讯作者：
Compant, Stephane
中科院分区：
文献类型：
--
作者：
del Barrio-Duque, Alejandro;Samad, Abdul;Compant, Stephane
植物宿主真菌和细菌内生菌的社区，建立一个复杂的网络的多方相互作用，但他们相互作用的机制知之甚少。一些真菌，如有益的菌根样真菌Serendipita（=Piriformospora）indica，可以通过细菌帮助建立，存活和定殖。虽然这种真菌含有根瘤菌作为内生菌，我们假设其他细菌也可能与真菌建立联系，并结合S。细菌可以促进植物生长和健康。方法采用S. indica和属于甲基杆菌属（Methylobacterium）、缓步动物属（Tardiphaga）、红腹菌属（Rhodanaphyla）和Trinickia spp.的特点，在体外和他们对番茄生长和尖孢镰刀菌和立枯丝核菌的生物防治的效果。基于基因组和显微镜分析，使用荧光标记标记的真菌和细菌菌株描述了这些相互作用背后的可能机制。结果所有细菌均能刺激S.离体生长。此外，几种细菌刺激番茄植株生长，但与S。indica和细菌的表现并不比单一接种更好。首先，S. indica和细菌显著降低了真菌病原体的疾病进展。这些微生物似乎在根定殖的过程中合作，例如通过增加真菌孢子形成和菌丝扩张，显示微生物和植物之间的多方相互作用。有趣的是，Trinickia菌株在内部定殖S. indica作为一个内生真菌细菌在体外共培养过程中，进一步表明，真菌可能会获得以前未识别的细菌属和细菌的基因组分析揭示了许多基因可能参与真菌和植物生长刺激，生物控制和根定殖，突出植物-真菌-细菌相互作用的推定机制。结论我们的研究为揭示植物间复杂的相互作用迈出了重要的一步。结果表明，在真菌接种物中添加细菌对植物S.籼型共生
Aims Plants host communities of fungal and bacterial endophytes, establishing a complex network of multipartite interactions, but the mechanisms whereby they interact are poorly understood. Some fungi, such as the beneficial mycorrhiza-like fungus Serendipita (=Piriformospora) indica, can be helped by bacteria for establishment, survival and colonization. Although this fungus harbors a Rhizobium as an endofungal bacterium, we hypothesized that other bacteria might also establish associations with the fungus and combining S. indica with bacteria might enhance plant growth and health. Methods The interactions among S. indica and four endophytic Proteobacteria belonging to Methylobacterium, Tardiphaga, Rhodanobacter and Trinickia spp. were characterized in vitro and for their effect on tomato growth and biocontrol of Fusarium oxysporum and Rhizoctonia solani. Possible mechanisms behind these interactions were described based on genome and microscopic analyses, using fungal and bacterial strains tagged with fluorescent markers. Results All bacteria stimulated S. indica growth in vitro. Moreover, several of the bacteria stimulated growth of tomato plants, but co-inoculations with S. indica and bacteria did not perform better than single inoculations. Contrarily, combinations of S. indica and bacteria significantly reduced disease progression of fungal pathogens. These microbes seem to cooperate in the process of root colonization for instance by increasing fungal sporulation and hyphae expansion, showing multipartite interaction between microbes and plants. Interestingly, the strain of Trinickia internally colonizes spores of S. indica as an endofungal bacterium during in vitro-co-culturing, suggesting further that the fungus might acquire formerly unrecognized genera of bacteria and genome analysis of the bacteria revealed many genes potentially involved in fungal and plant growth stimulation, biocontrol and root colonization, highlighting putative mechanisms of plant-fungal-bacterial interaction. Conclusions Our study represents an important step towards unraveling the complex interactions among plants, S. indica, endophytic bacteria and fungal pathogens, and indicates that adding bacteria to fungal inoculum could have a remarkable impact on the plant-S. indica symbiosis.