Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone.
Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone.
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DOI：
10.1038/s41598-017-03240-8
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发表时间：
2017-06-08
影响因子：
4.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Fu L;Huang M;Han B;Sun X;Sree KS;Appenroth KJ;Zhang J
浮萍有很大的潜力发展成为快速生长的作物，用于水修复和生物能源生产。在这一过程中，制种和利用杂种优势是必不可少的步骤。然而，即使在被广泛研究的浮萍物种中，浮萍的花原基也往往在成熟之前就夭折了。水杨酸(SA)和琼脂固化促进了吉巴7741和5504的成花，开花率较高。与吉巴5504不同，吉巴7741个体间的人工异花授粉产生的种子频率较高。与7741相比，5504的花药成熟后不开裂，其人工释放的花粉粒具有菠萝状的外壁和倾斜的刺。这些花粉不被2，5-二苯基单四氮溴(MTT)染色，不能萌发。因此，5504是雄性不育的，在杂种优势方面具有潜在的应用前景。此外，用7741个花粉粒授粉的5504花产生了种内杂交种子，这一点得到了ISSR标记的证实。这些杂交种子以高频率萌发，形成新的无性系。
Duckweed species have a great potential to develop into fast-growing crops for water remediation and bioenergy production. Seed production and utilization of hybrid vigour are essential steps in this process. However, even in the extensively-studied duckweed species, Lemna gibba, flower primordia were often aborted prior to maturation. Salicylic acid (SA) and agar solidification of the medium promoted flower maturation and resulted in high flowering rates in L. gibba 7741 and 5504. Artificial cross-pollination between individuals of L. gibba 7741 yielded seeds at high frequencies unlike that in L. gibba 5504. In contrast to clone 7741, the anthers of 5504 did not dehisce upon maturation, its artificially released pollen grains had pineapple-like exine with tilted spines. These pollens were not stained by 2,5-diphenylmonotetrazoliumbromide (MTT) and failed to germinate. Therefore, clone 5504 is male sterile and has potential application with respect to hybrid vigour. Moreover, pollination of flowers of 5504 with 7741 pollen grains resulted in intraspecific hybrid seeds, which was confirmed by inter-simple sequence repeat (ISSR) markers. These hybrid seeds germinated at a high frequency, forming new clones.