Quantitative trait loci mapping of functional traits in the German Holstein cattle population
Quantitative trait loci mapping of functional traits in the German Holstein cattle population
复制标题
DOI：
10.3168/jds.s0022-0302(03)73614-5
复制
发表时间：
2003-01-01
影响因子：
3.5
通讯作者：
Kalm, E
中科院分区：
文献类型：
--
作者：
Kühn, C;Bennewitz, J;Kalm, E
对德国荷斯坦牛群体进行了全基因组扫描，以检测功能性状的数量性状基因座（QTL）。为此，在16个孙女设计的家庭中，872个儿子的所有常染色体和性染色体的假常染色体区域的263个遗传标记进行了基因分型。调查的性状是母体和直接影响难产（DYS（m），DYS（d））和死胎（STI（m），STI（d））的负回归育种值，以及母体和父亲对90 d不归率（NR 90（m），NR 90（p））的影响。此外，退化育种值的功能畜群寿命（FHL）和女儿的体细胞计数（SCC）的产量偏差进行了调查。应用家系间加权多标记回归分析和排列检验检测QTL并计算统计显著性。DYS（m）和SCC分别在8号染色体和18号染色体上定位了10%的基因组显著性QTL。检测到另外24个超过5%染色体阈值的推定QTL。在第7、8、10、18号染色体和X/Y（ps）上，多个性状的QTL同时存在。我们的研究结果表明，影响乳房健康的基因座也可能有助于长寿的遗传变异。在实施这些QTL的标记辅助选择程序的功能性状，这些QTL的直接和相关的影响，以及精细定位的染色体位置的信息是必需的。
A whole-genome scan to detect quantitative trait loci (QTL) for functional traits was performed in the German Holstein cattle population. For this purpose, 263 genetic markers across all autosomes and the pseudoautosomal region of the sex chromosomes were genotyped in 16 granddaughter-design families with 872 sons. The traits investigated were deregressed breeding values for maternal and direct effects on dystocia (DYS(m), DYS(d)) and stillbirth (STI(m), STI(d)) as well as maternal and paternal effects on nonreturn rates of 90 d (NR90(m), NR90(p)). Furthermore, deregressed breeding values for functional herd life (FHL) and daughter yield deviation for somatic cell count (SCC) were investigated. Weighted multimarker regression analyses across families and permutation tests were applied for the detection of QTL and the calculation of statistical significance. A ten percent genomewise significant QTL was localized for DYS(m) on chromosome 8 and for SCC on chromosome 18. A further 24 putative QTL exceeding the 5% chromosomewise threshold were detected. On chromosomes 7, 8, 10, 18, and X/Y(ps), coincidence of QTL for several traits was observed. Our results suggest that loci with influence on udder health may also contribute to genetic variance of longevity. Prior to implementation of these QTL in marker assisted selection programs for functional traits, information about direct and correlated effects of these QTL as well as fine mapping of their chromosomal positions is required.