5 NEW INSULIN-PRODUCING CELL-LINES WITH DIFFERING SECRETORY PROPERTIES
5 NEW INSULIN-PRODUCING CELL-LINES WITH DIFFERING SECRETORY PROPERTIES
复制标题
DOI：
10.1677/joe.0.1090193
复制
发表时间：
1986-05-01
影响因子：
4
通讯作者：
HALES, CN
中科院分区：
文献类型：
--
作者：
CARRINGTON, CA;RUBERY, ED;HALES, CN
Five cell lines have been derived from a rat transplantable islet cell tumour using two different methods. The lines differ in morphology and contain and release different amounts of insulin and glucagon (insulin content, 1-90 pmol/106 cells; insulin release, 6-250 pmol/106 cells per 24 h; glucagon content, < 0.005-35 pmol/106 cells; glucagon release, < 0.05-10 pmol/106 cells per 24 h). All the lines responded to the presence of the secretagogues leucine (20 mmol/l) plus theophylline (5 mmol/l) by increasing the rate of release of insulin approximately twofold. A high extracellular concentration of potassium (40 mmol/l) caused a three- to tenfold calcium-dependent increase in release of insulin and a parallel release of glucagon. Increasing the concentration of glucose from 2.8 to 16.7 mmol/l did not alter the rate of insulin release by any of the cell lines.
