Burkholderia dilworthii sp nov., isolated from Lebeckia ambigua root nodules
Burkholderia dilworthii sp nov., isolated from Lebeckia ambigua root nodules
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DOI：
10.1099/ijs.0.058602-0
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发表时间：
2014-04-01
影响因子：
2.8
通讯作者：
Howieson, John G.
中科院分区：
文献类型：
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作者：
De Meyer, Sofie E.;Cnockaert, Margo;Howieson, John G.
从双歧Lebeckia ambigua根瘤中分离到3株革兰氏阴性杆状细菌，并对其进行鉴定。根据16S rRNA基因序列系统发育分析，它们均属于伯克霍尔德菌属，其中代表菌株WSM3556(T)与caledonica伯克霍尔德菌LMG 23644(T) （16S rRNA基因序列相似性为98.70%）和rhynchosiae伯克霍尔德菌WSM3937(T)（98.50%）亲缘关系最为密切。此外，这些菌株在管家基因gyrB和recA的系统发育树上形成了一个独特的群体。化学分类数据，包括脂肪酸谱和呼吸醌类分析，支持我们的菌株归属于伯克霍尔德菌属。DNA-DNA杂交、MALDI-TOF MS分析和生理生化测试的结果表明，我们的菌株与它们最近的邻居物种存在基因型和表型分化。因此，这些菌株代表了一个新种，建议将其命名为Burkholderia dilworthii sp. nov.，类型菌株为W5M3556(T) (=LMG 27173（T)=HAMBI 3353(T)）。
Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556(T) being most closely related to Burkholderia caledonica LMG 23644(T) (98.70% 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937(T) (98.50%). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain W5M3556(T) (=LMG 27173(T)=HAMBI 3353(T)).