Dynamic Histone Acetylation of Late Embryonic Genes during Seed Germination
Dynamic Histone Acetylation of Late Embryonic Genes during Seed Germination
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DOI：
10.1007/s11103-005-2081-x
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发表时间：
2005-12
影响因子：
5.1
通讯作者：
H. Tai;G. Tai;T. Beardmore
中科院分区：
文献类型：
--
作者：
H. Tai;G. Tai;T. Beardmore
组蛋白乙酰化参与植物和真核生物基因表达的调控。组蛋白脱乙酰酶（HDAC）是催化从组蛋白中去除乙酰基的酶，这与基因表达的抑制有关。为了研究组蛋白乙酰化在种子萌发过程中基因表达调控中的作用，用组蛋白去乙酰化酶的特异性抑制剂阿司他丁A（TSA）处理吸胀的拟南芥种子。基因芯片分析表明，TSA诱导种子萌发过程中45个基因表达上调，27个基因表达下调。筛选出8个TSA表达上调的基因--RAB 18、RD 29 B、ATEM 1、HSP 70和4个胚胎发育后期丰富蛋白基因（莱亚）。基因表达时间进程表明，这八个基因在干种子中以高水平表达，并在种子吸胀后以指数速率受到抑制。在TSA的存在下，八个基因的阻遏的开始不受影响，但阻遏表达的最终水平升高。染色质免疫沉淀和HDAC分析表明，有一个短暂的组蛋白脱乙酰化事件在种子萌发在吸胀后1天，这是一个关键的发育信号，影响的八个基因的镇压。
Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibingArabidopsis thalianaseeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis –RAB18,RD29B,ATEM1,HSP70and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at 1 day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes.