HUMAN FAD-DEPENDENT NAD(P)H DIAPHORASE
HUMAN FAD-DEPENDENT NAD(P)H DIAPHORASE
复制标题
DOI：
10.1042/bj1870429
复制
发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
HOPKINSON, DA
中科院分区：
文献类型：
--
作者：
EDWARDS, YH;POTTER, J;HOPKINSON, DA
一种新发现的人心肌黄酶，命名为心肌黄酶-4，它占大多数组织的心肌黄酶活性的主要部分，但不存在于红细胞，被描述。与其他人心肌黄酶相比，它在电泳后依赖于FAD的活性，被低浓度的双香豆素抑制，并对汽巴蓝显示出显着的亲和力。估计MW为49，000 +-。1800通过凝胶过滤。心肌黄酶-4似乎显示出人与人之间的数量差异，因此约4%的人群缺乏明显的酶活性，但尚不清楚这种变化是遗传还是非遗传起源。
A newly discovered human diaphorase, designated diaphorase-4, which accounts for a major part of the diaphorase activity of most tissues but does not occur in erythrocytes, is described. In contrast with other human diaphorases, it is dependent on FAD for activity after electrophoresis, inhibited by low concentrations of dicoumarol and shows a marked affinity for Cibacron Blue. The MW was estimated to be 49,000 .+-. 1800 by gel filtration. Diaphorase-4 appears to show person-to-person quantitative variation, so that about 4% of the population lack appreciable enzyme activity, but it is not yet clear whether this variation is of genetic or non-genetic origin.