PeSTZ1 confers salt stress tolerance by scavenging the accumulation of ROS through regulating the expression of PeZAT12 and PeAPX2 in Populus
PeSTZ1 confers salt stress tolerance by scavenging the accumulation of ROS through regulating the expression of PeZAT12 and PeAPX2 in Populus
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PeSTZ1 通过调节杨中 PeZAT12 和 PeAPX2 的表达来清除 ROS 的积累，从而赋予盐胁迫耐受性
DOI：
10.1093/treephys/tpaa050
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发表时间：
2020-09-01
期刊：
影响因子：
4
通讯作者：
Xia, Xinli
中科院分区：
文献类型：
--
作者：
He, Fang;Niu, Meng-Xue;Xia, Xinli
拟南芥ZAT12(ZAT12)在胁迫反应中起重要作用，但ZAT12在非生物胁迫下的转录调控尚不清楚。本研究通过双荧光素酶报告(DLR)实验和凝胶迁移率改变实验，证实了耐盐胡杨FINGER1转录因子(PeSTZ1)能够调控PeZAT12的表达。NaCI和过氧化氢(H_2O_2)处理能迅速诱导PeSTZ1的表达。高表达PeSTZ1基因的84K杨植株对盐胁迫具有较强的耐性。在盐胁迫下，转基因杨树比野生型杨树表现出更高的PeZAT12表达水平和更多的抗氧化剂积累。同时，抗坏血酸PEROX1Dase 2(PeAPX2)可被PeZAT12和PeSTZ1激活，促进细胞内抗坏血酸过氧化物酶(APX)的积累，清除盐胁迫下的活性氧(ROS)。这一新的调控模式(PeSTZ1-PeZAT12-PeAPX2)在杨树中被发现，为解释杨树抗性提供了新的思路和见解。转基因杨树减少了ROS的积累，抑制了叶绿素的降解，保证了光合作用和电子传递系统。另一方面，转基因杨树巧妙地调节K+/Na+动态平衡，缓解盐胁迫下植物光合器官的盐害，从而增加生物量的积累。综上所述，PeSTZI通过调节PeZAT12和PeAPX2的表达来清除ROS在杨树体内的积累，从而赋予杨树耐盐性。
ZINC FINGER OF ARABIDOPSIS THALIANA12 (ZAT12) plays an important role in stress responses, but the transcriptional regulation of ZAT1 2 in response to abiotic stress remains unclear. In this study, we confirmed that a SALT TOLERANCE ZINC FINGER1 transcription factor from Populus euphratica (PeSTZ1) could regulate the expression of PeZAT12 by dual-luciferase reporter (DLR) assay and electrophoretic mobility shift assay. The expression of PeSTZ1 was rapidly induced by NaCI and hydrogen peroxide (H2O2) treatments. Overexpressing PeSTZ1 in poplar 84K (Populus alba x Populus glandulosa) plant was endowed with a strong tolerance to salt stress. Under salt stress, transgenic poplar exhibited higher expression levels of PeZAT12 and accumulated a larger amount of antioxidant than the wild-type plants. Meanwhile, ASCORBATE PEROX1DASE2 (PeAPX2) can be activated by PeZAT12 and PeSTZ1, promoting the accumulation of cytosolic ascorbate peroxidase (APX) to scavenge reactive oxygen species (ROS) under salt stress. This new regulatory model (PeSTZ1-PeZAT12-PeAPX2) was found in poplar, providing a new idea and insight for the interpretation of poplar resistance. Transgenic poplar reduced the accumulation of ROS, restrained the degradation of chlorophyll and guaranteed the photosynthesis and electron transport system. On the other hand, transgenic poplar slickly adjusted K+/Na+ homeostasis to alleviate salt toxicity in photosynthetic organs of plants under salt stress and then increased biomass accumulation. In summary, PeSTZI confers salt stress tolerance by scavenging the accumulation of ROS through regulating the expression of PeZAT12 and PeAPX2 in poplar.