Field-based evaluation of a reagent strip test for diagnosis of Schistosoma mansoni by detecting circulating cathodic antigen in urine before and after chemotherapy
Field-based evaluation of a reagent strip test for diagnosis of Schistosoma mansoni by detecting circulating cathodic antigen in urine before and after chemotherapy
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DOI：
10.1016/j.trstmh.2006.11.009
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发表时间：
2007-07-01
影响因子：
2.2
通讯作者：
Erko, Berhanu
中科院分区：
文献类型：
--
作者：
Legesse, Mengistu;Erko, Berhanu
使用从埃塞俄比亚曼氏血吸虫流行区收集的 251 份粪便和尿液样本，在现场条件下评估了通过检测尿液中循环阴极抗原 (CCA) 来诊断血吸虫病的试剂条测试的敏感性。该测试的特异性是在血吸虫病非流行地区进行评估的。使用重复的加藤载玻片和甲醛-乙醚浓缩方法对粪便样本进行显微镜检查。化疗后还评估了该测试在监测疗效方面的有效性。结果显示，一步式试纸条检测尿液中CCA的检出率优于粪便检查法（P < 0.05），表明该疾病的患病率。假设结合寄生虫学检测结果作为金标准，该检测的敏感性和特异性分别为82.1%和75.9%。鸡蛋计数的结果表明，尿液 CCA 可以替代寄生虫学方法来指示感染强度。化疗后该测试的敏感性和特异性分别为 75% 和 73.7%。使用试剂条诊断尿液中的曼氏沙门氏菌感染将提供有关该疾病流行情况的信息，尽管需要进一步研究以提高其敏感性和特异性。 (C) 2007 年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
The sensitivity of a reagent strip test for the diagnosis of schistosomiasis by detecting circulating cathodic antigen (CCA) in urine was evaluated under field conditions using 251 stool and urine samples collected from a Schistosoma mansoni-endemic area of Ethiopia. The specificity of the test was evaluated in an area where schistosomiasis is not endemic. Stool samples were examined microscopically using duplicate Kato slides and formol-ether concentration methods. The effectiveness of the test in monitoring efficacy was also evaluated following chemotherapy. The results revealed that detection of CCA in urine using the one-step reagent strip test was superior to the stool examination methods (P < 0.05) in indicating the prevalence of the disease. Assuming the combination of parasitological test results as the gold standard, the sensitivity and specificity of the test were 82.1% and 75.9%, respectively. The results of egg counts suggested the potential use of urine CCA in indicating the intensity of infection as an alternative to parasitological methods. The sensitivity and specificity of the test were 75% and 73.7%, respectively, following chemotherapy. Diagnosis of S. mansoni infection in urine using reagent strips would provide information on the prevalence of the disease, although further study is needed to improve its sensitivity and specificity. (C) 2007 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.