Differential repair of UV damage in Saccharomyces cerevisiae.
Differential repair of UV damage in Saccharomyces cerevisiae.
复制标题
酿酒酵母紫外线损伤的差异修复。
DOI：
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发表时间：
1989
影响因子：
14.9
通讯作者：
P. Van de Putte
中科院分区：
文献类型：
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作者：
C. Terleth;C. V. van Sluis;P. Van de Putte
优先修复紫外线诱导的损伤是一种现象，哺乳动物细胞可能会提高其生存。本文提出的第一个证据表明，优先修复发生在低等真核生物酿酒酵母。此外，报道了一种独特的方法来比较存在于相同染色体上的相同序列，仅表达不同。我们确定了从两个相同的α交配型基因座中去除嘧啶二聚体，并且我们能够表明活性MAT α基因座优先于非活性HML α基因座修复。在sir-3突变体中，其中两个基因座都是活跃的，这种偏好没有观察到。
Preferential repair of UV-induced damage is a phenomenon by which mammalian cells might enhance their survival. This paper presents the first evidence that preferential repair occurs in the lower eukaryote Saccharomyces cerevisiae. Moreover an unique approach is reported to compare identical sequences present on the same chromosome and only differing in expression. We determined the removal of pyrimidine dimers from two identical alpha-mating type loci and we were able to show that the active MAT alpha locus is repaired preferentially to the inactive HML alpha locus. In a sir-3 mutant, in which both loci are active this preference is not observed.