Detection and identification of aqueous saccharides by using surface-enhanced Raman spectroscopy
Detection and identification of aqueous saccharides by using surface-enhanced Raman spectroscopy
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DOI：
10.1021/ac020115g
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发表时间：
2002-08-15
影响因子：
7.4
通讯作者：
Weaver, MJ
中科院分区：
文献类型：
--
作者：
Mrozek, MF;Weaver, MJ
通过基于表面增强拉曼光谱的策略的碳水化合物的灵敏检测和表征被证明。在粗糙的银基底上注入少量糖溶液，随后沉积银胶体后获得光谱。通过这种两步方法获得的灵敏度使得能够利用最小的激光功率和小的信号采集时间（几秒）对于少量的水溶液（5穆尔的10（-2）M溶液）获得高质量的拉曼光谱。光谱“指纹”获得的7个结构相似的单糖清楚地表明，每一种糖可以识别的有效手段。更复杂的分析的应用程序被证明为单糖混合物和二糖，其中的Sers指纹有助于成分的测定。
The sensitive detection and characterization of carbohydrates by means of a strategy based on surface-enhanced Raman spectroscopy is demonstrated. Spectra are obtained after injecting a small amount of saccharide solution onto a roughened silver substrate, with subsequent deposition of silver colloid. The sensitivity achieved by this two-step approach enables high-quality Raman spectra to be obtained for small amounts of aqueous saccharides (5 muL of a 10(-2) M solution) utilizing minimal laser power and small signal acquisition times (a few seconds). Spectral "fingerprints" obtained for seven structurally similar monosaccharides demonstrate clearly an effective means by which each sugar can be identified. The application to more complex analyses is demonstrated for monosaccharide mixtures and a disaccharide, whereby the SERS fingerprints aid in the determination of components.