AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
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DOI：
10.1590/s1415-47572004000100020
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发表时间：
2004-01-01
影响因子：
2.1
通讯作者：
Margis-Pinheiro, Márcia
中科院分区：
文献类型：
--
作者：
Gerhardt, Liliane B. de A.;Magioli, Cláudia;Margis-Pinheiro, Márcia
分析了AtitIV基因在非生物胁迫下拟南芥植物中的表达。在紫外光照射、外源水杨酸处理和伤害的响应下，叶片中检测到转录的积累。携带AtitIV启动子的转基因拟南芥植株：GUS融合也显示了报告基因在这些处理下的差异表达。并对拟南芥胚胎发育过程中AtitIV的表达进行了分析。GUS检测表明，从鱼雷期到完全成熟的合子胚胎中，AtitIV启动子处于激活状态。启动子缺失分析表明，所有负责特定组织表达的5‘顺式作用元件都位于转录起始附近1083bp的区域。还观察到位于-1083和-600之间的负调控区域。
The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter: gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV premoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed.