Transplacental transmission of a North American isolate of Leishmania infantum in an experimentally infected beagle
Transplacental transmission of a North American isolate of Leishmania infantum in an experimentally infected beagle
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DOI：
10.1645/ge-483r.1
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发表时间：
2005-08-01
影响因子：
1.3
通讯作者：
Lindsay, DS
中科院分区：
文献类型：
--
作者：
Rosypal, AC;Troy, GC;Lindsay, DS
婴儿利什曼原虫是人畜共患内脏利什曼病的病原体，在美国的猎狐犬中广泛传播。虽然白蛉在美国广泛分布，但流行病学资料并不支持白蛉在L。在这个国家的猎狐犬中。人类内脏利什曼病的先天性传播在人类中有报道，也可能发生在狗中。我们以前分离出L。来自弗吉尼亚猎狐犬的infantum，并使用该分离物（LIVT-1）实验感染比格犬。将4只慢性感染LIVT-I的雌性比格犬与1只慢性感染LIVT-I的雄性比格犬交配。chagasi婴儿一只比格犬能够维持妊娠，4只幼犬通过剖腹产分娩。一只幼犬在分娩时畸形和自溶，未收集或分析组织。其余幼犬在剖腹产时处死，收集选定组织进行寄生虫培养和PCR。未从任何幼犬的组织中培养前鞭毛体。利什曼原虫属DNA通过PCR在所有3只幼犬的肝脏、骨髓和心脏以及2只幼犬的脾脏、淋巴结、肾脏和胎盘中可检测到。母体胎盘组织为PCR阴性。这是第一次报告的母亲传播的一个北美分离的L。从实验感染的狗身上取下的婴儿
Leishmania infantum, an etiologic agent of zoonotic visceral leishmaniasis, is widespread among foxhounds in the United States. Although sand flies are widely distributed throughout the United States, epidemiological data do not support a major role for sand flies in the transmission of L. infantum in foxhounds in this country. Congenital transmission of human visceral leishmaniasis is reported in humans and might also occur in dogs. We have previously isolated L. infantum from Virginia foxhounds and used this isolate (LIVT-1) to experimentally infect beagles. Four female beagles, chronically infected with LIVT-I, were bred to a male beagle chronically infected with L. infantum chagasi. One beagle was able to maintain her pregnancy, and 4 puppies were delivered by cesarean section. One puppy was malformed and autolytic at delivery, and tissues were not collected or analyzed. The remaining puppies were killed at the time of cesarean section, and selected tissues were collected for parasite culture and PCR. Promastigotes were not cultured from tissues in any of the puppies. Leishmania sp. DNA was detectable by PCR in liver, bone marrow, and heart from all 3 puppies and in the spleen, lymph node, kidney, and placenta in 2 puppies. Placental tissue from the dam was PCR negative. This is the first report of maternal transmission of a North American isolate of L. infantum from an experimentally infected dog.