Plant-derived anti-inflammatory compounds affect MIF tautomerase activity
Plant-derived anti-inflammatory compounds affect MIF tautomerase activity
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DOI：
10.1016/j.intimp.2004.12.017
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发表时间：
2005-05-01
影响因子：
5.6
通讯作者：
Garai, J
中科院分区：
文献类型：
--
作者：
Molnar, V;Garai, J
近年来，细胞因子巨噬细胞移动抑制因子(MIF)已成为类风湿关节炎(RA)发病机制中的重要因子。关于MIF介导的苯丙酮酸或其其他酚类底物的互变异构转换是否与该细胞因子的促炎作用有关尚有争议。几个世纪以来，传统的草药疗法一直被用来缓解包括关节炎在内的多种炎症性疾病。它们的几种活性成分被鉴定为单酚或多酚衍生物。本文研究了一些抗炎植物酚对MIF介导的苯丙酮酸互变异构化的影响。姜黄素和咖啡酸被发现是最有效的抑制剂，在酮酶试验中显示出亚微摩尔范围的IC50值。白藜芦醇和伞形素几乎和解热止痛药对乙酰氨基酚一样有效。我们的结果表明，MIF可能是中草药抗风湿剂的靶点。(C)2005爱思唯尔B.V.保留所有权利。
The cytokine macrophage migration inhibitory factor (MIF) has recently emerged as a crucial factor in the pathogenesis of rheumatoid arthritis (RA). It is debated whether the MIF mediated tautomeric conversion of either phenylpyruvate or of its other phenolic substrates is implicated in the pro-inflammatory action of this cytokine. Traditional herbal remedies have been used for centuries to alleviate inflammatory ailments of many kinds including arthritis. Several of their active ingredients identified are mono- or poly-phenol derivatives. In the present study the effect of some anti-inflammatory plant phenols on MIF mediated tautomerism of phenylpyruvate was investigated. Curcumin and caffeic acid were found to be the most potent inhibitors, exhibiting IC50 values in the submicromolar range in the ketonase assay. Resveratrol and umbelliferon were almost as potent inhibitors as the antipyretic-analgetic drug acetaminophen. Our results reveal MIF as a possible target for the herbal anti-rheumatic agents. (c) 2005 Elsevier B.V All rights reserved.