Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy
Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy
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DOI：
10.1016/0003-9969(95)00130-1
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发表时间：
1996-04-01
影响因子：
3
通讯作者：
Pashley, DH
中科院分区：
文献类型：
--
作者：
Carvalho, RM;Yoshiyama, M;Pashley, DH
牙本质棒测量约。0.7 x 0.7 x 5.0 mm的牙冠由提取的人第三磨牙的牙冠制备。将标本在0.5 M EDTA（pH 7.0）中脱矿3天，并在解剖显微镜下用数字测微计测量其体积。将标本随机分配到实验组，并在丙酮中进行化学脱水。接下来，使用六甲基二硅氮烷、Peldri II或临界点干燥技术将它们干燥。在每个步骤之后再次测量试样的尺寸，并且将体积变化表示为原始去矿物质体积的百分比。在用丙酮脱水之前将试样固定在10%缓冲福尔马林中的效果也针对每个干燥程序进行了研究。在丙酮中脱水导致一个小的，但显着减少的体积脱矿物质福尔马林固定的标本，但未固定的标本没有显着变化。一般来说，所有三种干燥程序都会导致脱矿牙本质样本出现一定程度的收缩。未固定的试样表现出约为100%的体积收缩。15-20%，用任何方法干燥后。干燥后，固定标本比未固定标本收缩更多（25-35%）。无论干燥技术如何，当在扫描电子显微镜的真空室中测量时，样品进一步收缩10-20%。在这三种干燥技术中，六甲基二硅氮烷似乎是一种非常有用的替代临界点干燥用于制备扫描电子显微镜的牙本质标本。版权所有（C）1996 Elsevier Science Ltd.
Dentine rods measuring approx. 0.7 x 0.7 x 5.0 mm were prepared from the crowns of extracted human third molars. The specimens were demineralized in 0.5 M EDTA (pH 7.0) for 3 days and their volume measured with a digital micrometer under a dissecting microscope. The specimens were randomly assigned to experimental groups and were chemically dehydrated in acetone. Next they were dried using either hexamethyldisilazane, Peldri II, or critical-point drying techniques. The dimensions of the specimens were measured again after each step and the changes in volume were expressed as a percentage of the original demineralized volume. The effects of fixing the specimens in 10% buffered formalin before dehydration with acetone were also investigated for every drying procedure. Dehydration in acetone caused a small but significant reduction in the volume of demineralized formalin-fixed specimens but unfixed specimens did not change significantly. In general, all three drying procedures caused some shrinkage in demineralized dentine specimens. Unfixed specimens exhibited a volumetric shrinkage of approx. 15-20% after drying with any of the methods. Fixed specimens shrank more than unfixed specimens after drying (25-35%). Regardless of the drying technique, the specimens shrank a further 10-20% when measured in the vacuum chamber of the scanning electron microscope. Among the three drying techniques employed, hexamethyldisilazane seems to be a very useful alternative to critical-point drying for the preparation of dentine specimens for scanning electron microscopy. Copyright (C) 1996 Elsevier Science Ltd.